Human CCL17/TARC ELISA Kit PicoKine®

Human CCL17/TARC ELISA Kit PicoKine® (96 Tests) . Quantitate Human CCL17 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-C motif) ligand 17 (CCL17) is a small cytokine belonging to the CC chemokine family that is also known as thymus and activation regulated chemokine (TARC) . CCL17 is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells.1 This chemokine specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4.1,2The gene for CCL17 is located on chromosome 16, in humans, along with other chemokines called CCL22 and CX3CL1.3,4 The standard used in this kit is recombinant human CCL17, consisting of 71 amino acids with the molecular weight of 8Kda.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 62 58 60 57 59 1.92 3.2% Sample 2 251 287 247 277 265 16.93 6.3% Sample 3 939 986 993 986 976 21.55 2.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 62 58 60 57 59 1.92 3.2% Sample 1 62 58 60 57 59 1.92 3.2% Sample 2 251 287 247 277 265 16.93 6.3% Sample 2 251 287 247 277 265 16.93 6.3% Sample 3 939 986 993 986 976 21.55 2.2% Sample 3 939 986 993 986 976 21.55 2.2%

Synonyms

C-C motif chemokine 17; CC chemokine TARC; Small-inducible cytokine A17; Thymus and activation-regulated chemokine; CCL17; SCYA17, TARC

Gene Name

CCL17

Gene ID

6361

UniProt

Q92583

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A24-S94

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed at high levels in thymus and at low levels in the lung, colon and small intestine.

Applications

ELISA

Field of Research

Chemokines, Immunology, Innate Immunity, Macrophage/Inflammation

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 62 251 939 56 234 931 Standard deviation 2.6 10.79 53.52 2.68 12.16 62.37 CV (%) 4.2% 4.3% 5.7% 4.8% 5.2% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 62 251 939 56 234 931 Standard deviation 2.6 10.79 53.52 2.68 12.16 62.37 CV (%) 4.2% 4.3% 5.7% 4.8% 5.2% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 62 251 939 56 234 931 Mean (pg/ml) 62 251 939 56 234 931 Standard deviation 2.6 10.79 53.52 2.68 12.16 62.37 Standard deviation 2.6 10.79 53.52 2.68 12.16 62.37 CV (%) 4.2% 4.3% 5.7% 4.8% 5.2% 6.7% CV (%) 4.2% 4.3% 5.7% 4.8% 5.2% 6.7%

Sensitivity

<1 pg/mL

Function

Chemotactic factor for T-lymphocytes but not monocytes or granulocytes. May play a role in T-cell development in thymus and in trafficking and activation of mature T-cells. Binds to CCR4. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

10507 MW

Specificity

Natural and recombinant human CCL17

Anticoagulant

Heparin or EDTA

Gene Name Synonym

C-C motif chemokine 17

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the intercrine beta (chemokine CC) family.

Protein Name

C-C motif chemokine 17

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.052 0.13 0.189 0.274 0.441 0.764 1.268 1.967 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.052 0.13 0.189 0.274 0.441 0.764 1.268 1.967 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.052 0.13 0.189 0.274 0.441 0.764 1.268 1.967 O.D. 0.052 0.13 0.189 0.274 0.441 0.764 1.268 1.967

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.