Mouse CCL7/MCP-3 ELISA Kit PicoKine®

Chemokine (C-C motif) ligand 7 (CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3 (MCP3) . It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 34 32 32 32 0.82 2.5% Sample 2 194 218 205 216 208 9.6 4.6% Sample 3 459 441 412 484 449 26.25 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 34 32 32 32 0.82 2.5% Sample 1 33 34 32 32 32 0.82 2.5% Sample 2 194 218 205 216 208 9.6 4.6% Sample 2 194 218 205 216 208 9.6 4.6% Sample 3 459 441 412 484 449 26.25 5.8% Sample 3 459 441 412 484 449 26.25 5.8%

C-C motif chemokine 7; Intercrine/chemokine MARC; Monocyte chemoattractant protein 3; Monocyte chemotactic protein 3; MCP-3; Protein FIC; Small-inducible cytokine A7; Ccl7; Fic, Mcp3, Scya7

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 194 459 31 176 492 Standard deviation 1.51 13.19 30.75 1.58 14.08 42.31 CV (%) 4.6% 6.8% 6.7% 8.6% 8% 8.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 194 459 31 176 492 Standard deviation 1.51 13.19 30.75 1.58 14.08 42.31 CV (%) 4.6% 6.8% 6.7% 8.6% 8% 8.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 33 194 459 31 176 492 Mean (pg/ml) 33 194 459 31 176 492 Standard deviation 1.51 13.19 30.75 1.58 14.08 42.31 Standard deviation 1.51 13.19 30.75 1.58 14.08 42.31 CV (%) 4.6% 6.8% 6.7% 8.6% 8% 8.6% CV (%) 4.6% 6.8% 6.7% 8.6% 8% 8.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.097 0.148 0.196 0.286 0.462 0.858 1.328 1.965 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.097 0.148 0.196 0.286 0.462 0.858 1.328 1.965 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.097 0.148 0.196 0.286 0.462 0.858 1.328 1.965 O.D. 0.097 0.148 0.196 0.286 0.462 0.858 1.328 1.965

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.