Mouse CCL7/MCP-3 ELISA Kit PicoKine®

Mouse CCL7/MCP-3 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Ccl7 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-C motif) ligand 7 (CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3 (MCP3) . It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 34 32 32 32 0.82 2.5% Sample 2 194 218 205 216 208 9.6 4.6% Sample 3 459 441 412 484 449 26.25 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 34 32 32 32 0.82 2.5% Sample 1 33 34 32 32 32 0.82 2.5% Sample 2 194 218 205 216 208 9.6 4.6% Sample 2 194 218 205 216 208 9.6 4.6% Sample 3 459 441 412 484 449 26.25 5.8% Sample 3 459 441 412 484 449 26.25 5.8%

Synonyms

C-C motif chemokine 7; Intercrine/chemokine MARC; Monocyte chemoattractant protein 3; Monocyte chemotactic protein 3; MCP-3; Protein FIC; Small-inducible cytokine A7; Ccl7; Fic, Mcp3, Scya7

Gene Name

CCL7

Gene ID

20306

UniProt

Q03366

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q24-P97

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Chemokines, Immunology, Innate Immunity, Macrophage/Inflammation

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 194 459 31 176 492 Standard deviation 1.51 13.19 30.75 1.58 14.08 42.31 CV (%) 4.6% 6.8% 6.7% 8.6% 8% 8.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 194 459 31 176 492 Standard deviation 1.51 13.19 30.75 1.58 14.08 42.31 CV (%) 4.6% 6.8% 6.7% 8.6% 8% 8.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 33 194 459 31 176 492 Mean (pg/ml) 33 194 459 31 176 492 Standard deviation 1.51 13.19 30.75 1.58 14.08 42.31 Standard deviation 1.51 13.19 30.75 1.58 14.08 42.31 CV (%) 4.6% 6.8% 6.7% 8.6% 8% 8.6% CV (%) 4.6% 6.8% 6.7% 8.6% 8% 8.6%

Sensitivity

<1 pg/mL

Function

Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity (By similarity) . .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

10999 MW

Specificity

Natural and recombinant mouse CCL7

Anticoagulant

Heparin or EDTA

Gene Name Synonym

C-C motif chemokine 7

Subcellular Location

Secreted.

Protein Name

C-C motif chemokine 7

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.097 0.148 0.196 0.286 0.462 0.858 1.328 1.965 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.097 0.148 0.196 0.286 0.462 0.858 1.328 1.965 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.097 0.148 0.196 0.286 0.462 0.858 1.328 1.965 O.D. 0.097 0.148 0.196 0.286 0.462 0.858 1.328 1.965

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.