Human CA9/Carbonic anhydrase 9 ELISA Kit PicoKine®

Carbonic anhydrase 9 (CA9/CAIX) is an enzyme that in humans is encoded by the CA9 gene. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene is mapped to 9p13.3. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 32 32 33 33 2.06 6.2% Sample 2 196 230 215 208 212 12.29 5.7% Sample 3 412 423 456 402 423 20.31 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 32 32 33 33 2.06 6.2% Sample 1 37 32 32 33 33 2.06 6.2% Sample 2 196 230 215 208 212 12.29 5.7% Sample 2 196 230 215 208 212 12.29 5.7% Sample 3 412 423 456 402 423 20.31 4.8% Sample 3 412 423 456 402 423 20.31 4.8%

Carbonic anhydrase 9;4.2.1.1; Carbonate dehydratase IX; Carbonic anhydrase IX; CA-IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; RCC-associated antigen G250; pMW1; CA9; G250, MN

Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 196 412 33 176 428 Standard deviation 2.84 10.97 27%3 2.77 11.61 29.96 CV (%) 7.7% 5.6% 6.1% 8.4% 6.6% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 196 412 33 176 428 Standard deviation 2.84 10.97 27%3 2.77 11.61 29.96 CV (%) 7.7% 5.6% 6.1% 8.4% 6.6% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 37 196 412 33 176 428 Mean (pg/ml) 37 196 412 33 176 428 Standard deviation 2.84 10.97 27%3 2.77 11.61 29.96 Standard deviation 2.84 10.97 27%3 2.77 11.61 29.96 CV (%) 7.7% 5.6% 6.1% 8.4% 6.6% 7% CV (%) 7.7% 5.6% 6.1% 8.4% 6.6% 7%

Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Nucleus . Nucleus, nucleolus . Cell membrane ; Single-pass type I membrane protein . Cell projection, microvillus membrane ; Single-pass type I membrane protein . Found on the surface microvilli and in the nucleus, particularly in nucleolus.

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.089 0.181 0.25 0.368 0.602 0.984 1.575 2.126 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.089 0.181 0.25 0.368 0.602 0.984 1.575 2.126 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.089 0.181 0.25 0.368 0.602 0.984 1.575 2.126 O.D. 0.089 0.181 0.25 0.368 0.602 0.984 1.575 2.126

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.