Human Cystatin C/CST3 ELISA Kit PicoKine®

Cystatin C or cystatin 3 (formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid (CSF) and of urine from patients with renal failure.1 Cystatin 3 has a low molecular weight (approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In humans, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases2 (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases3 (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes) . Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.4

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 687 650 665 676 669 13.68 2% Sample 2 2837 2995 2880 2796 2877 74.32 2.5% Sample 3 10374 8987 8970 10046 9594 626.6 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 687 650 665 676 669 13.68 2% Sample 1 687 650 665 676 669 13.68 2% Sample 2 2837 2995 2880 2796 2877 74.32 2.5% Sample 2 2837 2995 2880 2796 2877 74.32 2.5% Sample 3 10374 8987 8970 10046 9594 626.6 6.5% Sample 3 10374 8987 8970 10046 9594 626.6 6.5%

Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level) . Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland. .

Apoptosis, Atherosclerosis, Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Intracellular, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 687 2837 10374 647 2715 10112 Standard deviation 43.96 207.1 684.68 51.11 217.2 859.52 CV (%) 6.4% 7.3% 6.6% 7.9% 8% 8.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 687 2837 10374 647 2715 10112 Standard deviation 43.96 207.1 684.68 51.11 217.2 859.52 CV (%) 6.4% 7.3% 6.6% 7.9% 8% 8.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 687 2837 10374 647 2715 10112 Mean (pg/ml) 687 2837 10374 647 2715 10112 Standard deviation 43.96 207.1 684.68 51.11 217.2 859.52 Standard deviation 43.96 207.1 684.68 51.11 217.2 859.52 CV (%) 6.4% 7.3% 6.6% 7.9% 8% 8.5% CV (%) 6.4% 7.3% 6.6% 7.9% 8% 8.5%

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.04 0.118 0.154 0.285 0.43 0.792 1.322 2.032 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.04 0.118 0.154 0.285 0.43 0.792 1.322 2.032 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.04 0.118 0.154 0.285 0.43 0.792 1.322 2.032 O.D. 0.04 0.118 0.154 0.285 0.43 0.792 1.322 2.032

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.