Mouse Cathepsin D ELISA Kit PicoKine®

Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 844 838 787 752 805 37.89 4.7% Sample 2 1778 2039 1841 1903 1890 96.58 5.1% Sample 3 7768 8873 9192 9277 8777 601.98 6.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 844 838 787 752 805 37.89 4.7% Sample 1 844 838 787 752 805 37.89 4.7% Sample 2 1778 2039 1841 1903 1890 96.58 5.1% Sample 2 1778 2039 1841 1903 1890 96.58 5.1% Sample 3 7768 8873 9192 9277 8777 601.98 6.8% Sample 3 7768 8873 9192 9277 8777 601.98 6.8%

Cancer, Cell Adhesion Proteins, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Membrane Proteins, Neuroscience, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 844 1778 7768 898 1799 8034 Standard deviation 63.3 99.56 458.31 77.43 133.12 514.17 CV (%) 7.5% 5.6% 5.9% 8.5% 7.4% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 844 1778 7768 898 1799 8034 Standard deviation 63.3 99.56 458.31 77.43 133.12 514.17 CV (%) 7.5% 5.6% 5.9% 8.5% 7.4% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 844 1778 7768 898 1799 8034 Mean (pg/ml) 844 1778 7768 898 1799 8034 Standard deviation 63.3 99.56 458.31 77.43 133.12 514.17 Standard deviation 63.3 99.56 458.31 77.43 133.12 514.17 CV (%) 7.5% 5.6% 5.9% 8.5% 7.4% 6.4% CV (%) 7.5% 5.6% 5.9% 8.5% 7.4% 6.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.024 0.049 0.096 0.214 0.358 0.773 1.338 2.094 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.024 0.049 0.096 0.214 0.358 0.773 1.338 2.094 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.024 0.049 0.096 0.214 0.358 0.773 1.338 2.094 O.D. 0.024 0.049 0.096 0.214 0.358 0.773 1.338 2.094

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.