Human Cathepsin D ELISA Kit PicoKine®

Human Cathepsin D ELISA Kit PicoKine® (96 Tests) . Quantitate Human CTSD in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 189 190 216 201 199 10.88 5.4% Sample 2 1630 1733 1553 1567 1620 71 4.3% Sample 3 5138 5161 4737 4988 5006 168.91 3.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 189 190 216 201 199 10.88 5.4% Sample 1 189 190 216 201 199 10.88 5.4% Sample 2 1630 1733 1553 1567 1620 71 4.3% Sample 2 1630 1733 1553 1567 1620 71 4.3% Sample 3 5138 5161 4737 4988 5006 168.91 3.3% Sample 3 5138 5161 4737 4988 5006 168.91 3.3%

Synonyms

Cathepsin D;3.4.23.5; Cathepsin D light chain; Cathepsin D heavy chain; CTSD; CPSD

Gene Name

CTSD

Gene ID

1509

UniProt

P07339

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L21-L412

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed in the aorta extrcellular space (at protein level) . .

Applications

ELISA

Field of Research

Cancer, Cell Adhesion Proteins, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Membrane Proteins, Neuroscience, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 189 1630 5138 181 1712 5150 Standard deviation 13.6 91.28 339.1 13.75 111.28 427.45 CV (%) 7.2% 5.6% 6.6% 7.6% 6.5% 8.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 189 1630 5138 181 1712 5150 Standard deviation 13.6 91.28 339.1 13.75 111.28 427.45 CV (%) 7.2% 5.6% 6.6% 7.6% 6.5% 8.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 189 1630 5138 181 1712 5150 Mean (pg/ml) 189 1630 5138 181 1712 5150 Standard deviation 13.6 91.28 339.1 13.75 111.28 427.45 Standard deviation 13.6 91.28 339.1 13.75 111.28 427.45 CV (%) 7.2% 5.6% 6.6% 7.6% 6.5% 8.3% CV (%) 7.2% 5.6% 6.6% 7.6% 6.5% 8.3%

Sensitivity

<10 pg/mL

Function

Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

44552 MW

Specificity

Natural and recombinant human Cathepsin D

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Cathepsin D

Subcellular Location

Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380) . .

Sequence Similarities

Belongs to the peptidase A1 family.

Protein Name

Cathepsin D

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.009 0.083 0.143 0.259 0.504 0.832 1.434 2.26 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.009 0.083 0.143 0.259 0.504 0.832 1.434 2.26 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.009 0.083 0.143 0.259 0.504 0.832 1.434 2.26 O.D. 0.009 0.083 0.143 0.259 0.504 0.832 1.434 2.26

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.