Mouse Cathepsin B ELISA Kit PicoKine®

Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene.1, 2 And this gene is mapped to chromosome 8p22.3 The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease.4 Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant mouse Cathepsin B with the molecular mass of 37KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 242 214 229 235 230 10.31 4.4% Sample 2 1828 1771 1721 1761 1770 38.23 2.1% Sample 3 4749 4980 5227 4633 4897 227.69 4.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 242 214 229 235 230 10.31 4.4% Sample 1 242 214 229 235 230 10.31 4.4% Sample 2 1828 1771 1721 1761 1770 38.23 2.1% Sample 2 1828 1771 1721 1761 1770 38.23 2.1% Sample 3 4749 4980 5227 4633 4897 227.69 4.6% Sample 3 4749 4980 5227 4633 4897 227.69 4.6%

Angiogenesis, Cancer, Cell Adhesion, Cell Adhesion Proteins, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Membrane Proteins, Neuroscience, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 242 1828 4749 229 1916 4534 Standard deviation 14.76 100.54 370.42 13.96 128.37 439.79 CV (%) 6.1% 5.5% 7.8% 6.1% 6.7% 9.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 242 1828 4749 229 1916 4534 Standard deviation 14.76 100.54 370.42 13.96 128.37 439.79 CV (%) 6.1% 5.5% 7.8% 6.1% 6.7% 9.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 242 1828 4749 229 1916 4534 Mean (pg/ml) 242 1828 4749 229 1916 4534 Standard deviation 14.76 100.54 370.42 13.96 128.37 439.79 Standard deviation 14.76 100.54 370.42 13.96 128.37 439.79 CV (%) 6.1% 5.5% 7.8% 6.1% 6.7% 9.7% CV (%) 6.1% 5.5% 7.8% 6.1% 6.7% 9.7%

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.037 0.094 0.162 0.273 0.495 0.871 1.373 2.092 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.037 0.094 0.162 0.273 0.495 0.871 1.373 2.092 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.037 0.094 0.162 0.273 0.495 0.871 1.373 2.092 O.D. 0.037 0.094 0.162 0.273 0.495 0.871 1.373 2.092

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.