Human Cathepsin B ELISA Kit PicoKine®

Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene.1, 2 And this gene is mapped to chromosome 8p22.3 The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease.4 Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant human Cathepsin B with the molecular mass of 37KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 252 275 235 272 258 16.19 6.2% Sample 2 1856 1776 1691 1826 1787 62.48 3.4% Sample 3 4296 4210 3832 4617 4238 279.56 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 252 275 235 272 258 16.19 6.2% Sample 1 252 275 235 272 258 16.19 6.2% Sample 2 1856 1776 1691 1826 1787 62.48 3.4% Sample 2 1856 1776 1691 1826 1787 62.48 3.4% Sample 3 4296 4210 3832 4617 4238 279.56 6.5% Sample 3 4296 4210 3832 4617 4238 279.56 6.5%

Angiogenesis, Cancer, Cell Adhesion, Cell Adhesion Proteins, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Membrane Proteins, Neuroscience, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 252 1856 4296 234 1923 4064 Standard deviation 17.64 129.92 326.49 17.85 148.07 377.95 CV (%) 7% 7% 7.6% 7.8% 7.7% 9.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 252 1856 4296 234 1923 4064 Standard deviation 17.64 129.92 326.49 17.85 148.07 377.95 CV (%) 7% 7% 7.6% 7.8% 7.7% 9.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 252 1856 4296 234 1923 4064 Mean (pg/ml) 252 1856 4296 234 1923 4064 Standard deviation 17.64 129.92 326.49 17.85 148.07 377.95 Standard deviation 17.64 129.92 326.49 17.85 148.07 377.95 CV (%) 7% 7% 7.6% 7.8% 7.7% 9.3% CV (%) 7% 7% 7.6% 7.8% 7.7% 9.3%

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.094 0.126 0.192 0.327 0.56 1.055 1.926 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.094 0.126 0.192 0.327 0.56 1.055 1.926 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.094 0.126 0.192 0.327 0.56 1.055 1.926 O.D. 0.055 0.094 0.126 0.192 0.327 0.56 1.055 1.926

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.