Mouse BAFF ELISA Kit PicoKine®

B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFLSF13B gene.1,2 BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells.3All these ligands act as heterotrimers (i.e. three of the same molecule) interacting with heterotrimeric receptors4, although BAFF has been known to be active as either a hetero- or homotrimer.5 The standard used in this kit is recombinant mouse soluble BAFF (A127-L309) with molecular weight 23.2KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 82 85 90 80 84 3.76 4.4% Sample 2 293 335 318 295 310 17.34 5.5% Sample 3 1221 1161 1374 1312 1267 81.89 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 82 85 90 80 84 3.76 4.4% Sample 1 82 85 90 80 84 3.76 4.4% Sample 2 293 335 318 295 310 17.34 5.5% Sample 2 293 335 318 295 310 17.34 5.5% Sample 3 1221 1161 1374 1312 1267 81.89 6.4% Sample 3 1221 1161 1374 1312 1267 81.89 6.4%

Adaptive Immunity, B Cells, Cancer, Cytokines, Growth Factors, Growth Factors/Hormones, Immunology, Innate Immunity, Non-CD, Signal Transduction, TNF Superfamily

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 82 287 1221 76 287 1209 Standard deviation 6.47 15.52 67.15 7.14 16.07 72.54 CV (%) 7.9% 5.3% 5.5% 9.4% 5.6% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 82 287 1221 76 287 1209 Standard deviation 6.47 15.52 67.15 7.14 16.07 72.54 CV (%) 7.9% 5.3% 5.5% 9.4% 5.6% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 82 287 1221 76 287 1209 Mean (pg/ml) 82 287 1221 76 287 1209 Standard deviation 6.47 15.52 67.15 7.14 16.07 72.54 Standard deviation 6.47 15.52 67.15 7.14 16.07 72.54 CV (%) 7.9% 5.3% 5.5% 9.4% 5.6% 6% CV (%) 7.9% 5.3% 5.5% 9.4% 5.6% 6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.03 0.132 0.219 0.384 0.693 1.198 1.693 2.032 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.03 0.132 0.219 0.384 0.693 1.198 1.693 2.032 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.03 0.132 0.219 0.384 0.693 1.198 1.693 2.032 O.D. 0.03 0.132 0.219 0.384 0.693 1.198 1.693 2.032

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.