Human BAFF / TNFSF13B ELISA Kit PicoKine®

BAFF was regularly detected by enzyme-linked immunosorbent assay in brain tissue lysates and in normal spinal fluid, and in astrocytes by double fluorescence microscopy. BAFF was localized in astrocytes close to BAFF-R-expressing immune cells. BAFF receptors were strongly expressed in situ in primary central nervous system (CNS) lymphomas.1 The TNF superfamily member B cell-activating factor (BAFF) plays an important role in humoral immunity and in autoimmune diseases, including RA.Local BAFF gene targeting inhibited proinflammatory cytokine expression, suppressed generation of plasma cells and Th17 cells, and markedly ameliorated joint pathology.2 The B cell activating factor BAFF (BlyS/TALL-1/zTNF4) is a tumor necrosis factor (TNF) -related ligand that promotes B cell survival and binds to three receptors (BCMA, TACI, and the recently described BAFF-R) .3 Human BAFF was mapped to chromosome 13q32-34.4 The standard used in this kit is recombinant soluble human BAFF (A134-L295) with the molecular mass of 19.6KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 127 125 149 127 132 9.84 7.4% Sample 2 522 513 536 528 524 8.4 1.6% Sample 3 1229 1085 1215 1223 1188 59.67 5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 127 125 149 127 132 9.84 7.4% Sample 1 127 125 149 127 132 9.84 7.4% Sample 2 522 513 536 528 524 8.4 1.6% Sample 2 522 513 536 528 524 8.4 1.6% Sample 3 1229 1085 1215 1223 1188 59.67 5% Sample 3 1229 1085 1215 1223 1188 59.67 5%

Tumor necrosis factor ligand superfamily member 13B; B lymphocyte stimulator; BLyS; B-cell-activating factor; BAFF; Dendritic cell-derived TNF-like molecule; TNF- and APOL-related leukocyte expressed ligand 1; TALL-1; CD257; Tumor necrosis factor ligand superfamily member 13b, membrane form; Tumor necrosis factor ligand superfamily member 13b, soluble form; TNFSF13B; BAFF, BLYS, TALL1, TNFSF20, ZTNF4; UNQ401/PRO738

Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T- cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. Isoform 2 is expressed in many myeloid cell lines. .

Adaptive Immunity, B Cells, Cancer, Cytokines, Growth Factors, Growth Factors/Hormones, Immunology, Innate Immunity, Non-CD, Signal Transduction, TNF Superfamily

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 127 522 1229 124 548 1268 Standard deviation 5.46 38.62 79.88 5.7 47.67 103.97 CV (%) 4.3% 7.4% 6.5% 4.6% 8.7% 8.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 127 522 1229 124 548 1268 Standard deviation 5.46 38.62 79.88 5.7 47.67 103.97 CV (%) 4.3% 7.4% 6.5% 4.6% 8.7% 8.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 127 522 1229 124 548 1268 Mean (pg/ml) 127 522 1229 124 548 1268 Standard deviation 5.46 38.62 79.88 5.7 47.67 103.97 Standard deviation 5.46 38.62 79.88 5.7 47.67 103.97 CV (%) 4.3% 7.4% 6.5% 4.6% 8.7% 8.2% CV (%) 4.3% 7.4% 6.5% 4.6% 8.7% 8.2%

Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.063 0.139 0.201 0.381 0.626 1.102 1.615 1.872 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.063 0.139 0.201 0.381 0.626 1.102 1.615 1.872 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.063 0.139 0.201 0.381 0.626 1.102 1.615 1.872 O.D. 0.063 0.139 0.201 0.381 0.626 1.102 1.615 1.872

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.