Mouse TNFRSF17/BCMA ELISA Kit PicoKine®

Mouse TNFRSF17/BCMA ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Tnfrsf17 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

TNFRSF17 (Tumor necrosis factor receptor superfamily member 17), also called BCMA, is a protein that in humans is encoded by the TNFRSF17 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily and is mapped to 16p13.13. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B/TALL-1/BAFF), and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 82 85 83 96 86 5.59 6.5% Sample 2 288 282 296 300 291 6.98 2.3% Sample 3 757 861 852 799 817 42.08 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 82 85 83 96 86 5.59 6.5% Sample 1 82 85 83 96 86 5.59 6.5% Sample 2 288 282 296 300 291 6.98 2.3% Sample 2 288 282 296 300 291 6.98 2.3% Sample 3 757 861 852 799 817 42.08 5.1% Sample 3 757 861 852 799 817 42.08 5.1%

Synonyms

Tumor necrosis factor receptor superfamily member 17; B-cell maturation protein; CD269; Tnfrsf17; Bcm, Bcma

Gene Name

TNFRSF17

Gene ID

21935

UniProt

O88472

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-T49

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Detected in spleen, thymus, bone marrow and heart, and at lower levels in kidney and lung.

Applications

ELISA

Field of Research

Antiviral Signaling, Cancer, Growth Factors, Growth Factors/Hormones, Immune System Diseases, Immunology, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 82 288 757 87 276 787 Standard deviation 3.69 11.52 56.77 4.87 12.97 72.4 CV (%) 4.5% 4% 7.5% 5.6% 4.7% 9.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 82 288 757 87 276 787 Standard deviation 3.69 11.52 56.77 4.87 12.97 72.4 CV (%) 4.5% 4% 7.5% 5.6% 4.7% 9.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 82 288 757 87 276 787 Mean (pg/ml) 82 288 757 87 276 787 Standard deviation 3.69 11.52 56.77 4.87 12.97 72.4 Standard deviation 3.69 11.52 56.77 4.87 12.97 72.4 CV (%) 4.5% 4% 7.5% 5.6% 4.7% 9.2% CV (%) 4.5% 4% 7.5% 5.6% 4.7% 9.2%

Sensitivity

<10 pg/mL

Function

Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK (By similarity) . .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

20442 MW

Specificity

Natural and recombinant mouse TNFRSF17

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Tumor necrosis factor receptor superfamily member 17

Subcellular Location

Membrane ; Single-pass type III membrane protein .

Protein Name

Tumor necrosis factor receptor superfamily member 17

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.024 0.099 0.167 0.296 0.519 0.932 1.68 2.834 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.024 0.099 0.167 0.296 0.519 0.932 1.68 2.834 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.024 0.099 0.167 0.296 0.519 0.932 1.68 2.834 O.D. 0.024 0.099 0.167 0.296 0.519 0.932 1.68 2.834

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.