Human TNFRSF17/BCMA ELISA Kit PicoKine®

Human TNFRSF17/BCMA ELISA Kit PicoKine® (96 Tests) . Quantitate Human TNFRSF17 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

TNFRSF17 (Tumor necrosis factor receptor superfamily member 17), also called BCMA, is a protein that in humans is encoded by the TNFRSF17 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily and is mapped to 16p13.13. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B/TALL-1/BAFF), and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 64 58 60 62 61 2.23 3.6% Sample 2 237 213 221 231 225 9.2 4% Sample 3 917 840 916 821 873 43.52 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 64 58 60 62 61 2.23 3.6% Sample 1 64 58 60 62 61 2.23 3.6% Sample 2 237 213 221 231 225 9.2 4% Sample 2 237 213 221 231 225 9.2 4% Sample 3 917 840 916 821 873 43.52 4.9% Sample 3 917 840 916 821 873 43.52 4.9%

Synonyms

Tumor necrosis factor receptor superfamily member 17; B-cell maturation protein; CD269; TNFRSF17; BCM, BCMA

Gene Name

TNFRSF17

Gene ID

608

UniProt

Q02223

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-A54

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed in mature B-cells, but not in T- cells or monocytes.

Applications

ELISA

Field of Research

Antiviral Signaling, Cancer, Growth Factors, Growth Factors/Hormones, Immune System Diseases, Immunology, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 64 237 917 68 247 851 Standard deviation 4.09 13.98 42.18 5.57 16.54 43.4 CV (%) 6.4% 4.6% 4.6% 8.2% 6.7% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 64 237 917 68 247 851 Standard deviation 4.09 13.98 42.18 5.57 16.54 43.4 CV (%) 6.4% 4.6% 4.6% 8.2% 6.7% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 64 237 917 68 247 851 Mean (pg/ml) 64 237 917 68 247 851 Standard deviation 4.09 13.98 42.18 5.57 16.54 43.4 Standard deviation 4.09 13.98 42.18 5.57 16.54 43.4 CV (%) 6.4% 4.6% 4.6% 8.2% 6.7% 5.1% CV (%) 6.4% 4.6% 4.6% 8.2% 6.7% 5.1%

Sensitivity

<10 pg/mL

Function

Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

20165 MW

Specificity

Natural and recombinant human TNFRSF17

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Tumor necrosis factor receptor superfamily member 17

Subcellular Location

Cell membrane; Single-pass type III membrane protein. Endomembrane system; Single-pass type III membrane protein. Perinuclear Golgi-like structures.

Sequence Similarities

Contains 1 TNFR-Cys repeat.

Protein Name

Tumor necrosis factor receptor superfamily member 17

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.042 0.165 0.259 0.397 0.603 0.951 1.605 2.408 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.042 0.165 0.259 0.397 0.603 0.951 1.605 2.408 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.042 0.165 0.259 0.397 0.603 0.951 1.605 2.408 O.D. 0.042 0.165 0.259 0.397 0.603 0.951 1.605 2.408

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.