Human AXL ELISA Kit PicoKine®

Tyrosine-protein kinase receptor UFO is an enzyme that in humans is encoded by the AXL gene. The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, the Axl protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation. This receptor can also mediate cell aggregation by homophilic binding. Axl is a chronic myelogenous leukemia-associated oncogene and also associated with colon cancer and melanoma. It is in close vicinity to the bcl3 oncogene, which is at 19q13.1-q13.2. The Axl gene is evolutionarily conserved between vertebrate species. This gene has two different alternatively spliced transcript variants.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 103 88 85 85 90 7.46 8.2% Sample 2 593 706 654 601 638 45.47 7.1% Sample 3 1295 1325 1154 1246 1255 64.77 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 103 88 85 85 90 7.46 8.2% Sample 1 103 88 85 85 90 7.46 8.2% Sample 2 593 706 654 601 638 45.47 7.1% Sample 2 593 706 654 601 638 45.47 7.1% Sample 3 1295 1325 1154 1246 1255 64.77 5.1% Sample 3 1295 1325 1154 1246 1255 64.77 5.1%

Cancer, Cell Biology, Cell Cycle, Cell Type Markers, Kinases/Phosphatases, Oncoproteins, Oncoproteins/Suppressors, Protein Phosphorylation, Receptor Tyrosine Kinases, Signal Transduction, Tags & Cell Markers, Tumor Associated, Tyrosine Kinases

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 103 593 12107 107 550 1194 Standard deviation 8.13 45.06 86.76 9.3 49.5 86.7%6 CV (%) 7.9% 7.6% 6.7% 8.7% 9% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 103 593 12107 107 550 1194 Standard deviation 8.13 45.06 86.76 9.3 49.5 86.7%6 CV (%) 7.9% 7.6% 6.7% 8.7% 9% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 103 593 12107 107 550 1194 Mean (pg/ml) 103 593 12107 107 550 1194 Standard deviation 8.13 45.06 86.76 9.3 49.5 86.7%6 Standard deviation 8.13 45.06 86.76 9.3 49.5 86.7%6 CV (%) 7.9% 7.6% 6.7% 8.7% 9% 7.2% CV (%) 7.9% 7.6% 6.7% 8.7% 9% 7.2%

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3- kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs) -mediated innate immune response. In case of filovirus infection, seems to function as a cell entry factor. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.0 0.045 0.077 0.148 0.302 0.6255 1.173 1.985 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.0 0.045 0.077 0.148 0.302 0.6255 1.173 1.985 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.0 0.045 0.077 0.148 0.302 0.6255 1.173 1.985 O.D. 0.0 0.045 0.077 0.148 0.302 0.6255 1.173 1.985

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.