Human Angiopoietin-2 / ANG2 ELISA Kit PicoKine®

Angiopoietin-2 is a protein that in humans is encoded by the ANGPT2 gene. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. ANGPT2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. Its expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 327 351 360 317 338 17.41 5.1% Sample 2 1634 1902 1611 1928 1768 146.76 8.3% Sample 3 5900 6201 5314 5672 5771 324.1 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 327 351 360 317 338 17.41 5.1% Sample 1 327 351 360 317 338 17.41 5.1% Sample 2 1634 1902 1611 1928 1768 146.76 8.3% Sample 2 1634 1902 1611 1928 1768 146.76 8.3% Sample 3 5900 6201 5314 5672 5771 324.1 5.6% Sample 3 5900 6201 5314 5672 5771 324.1 5.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 327 1634 5900 341 1769 5438 Standard deviation 17.33 114.38 354 23.52 127.36 342.59 CV (%) 5.3% 7% 6% 6.9% 7.2% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 327 1634 5900 341 1769 5438 Standard deviation 17.33 114.38 354 23.52 127.36 342.59 CV (%) 5.3% 7% 6% 6.9% 7.2% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 327 1634 5900 341 1769 5438 Mean (pg/ml) 327 1634 5900 341 1769 5438 Standard deviation 17.33 114.38 354 23.52 127.36 342.59 Standard deviation 17.33 114.38 354 23.52 127.36 342.59 CV (%) 5.3% 7% 6% 6.9% 7.2% 6.3% CV (%) 5.3% 7% 6% 6.9% 7.2% 6.3%

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.023 0.112 0.198 0.318 0.611 1.1 1.696 2.017 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.023 0.112 0.198 0.318 0.611 1.1 1.696 2.017 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.023 0.112 0.198 0.318 0.611 1.1 1.696 2.017 O.D. 0.023 0.112 0.198 0.318 0.611 1.1 1.696 2.017

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.