Human Endoglin/CD105/ENG ELISA Kit PicoKine®

CD105, also called Endoglin, is a homodimeric membrane glycoprotein primarily associated with human vascular endothelium. It is also found on bone marrow proerythroblasts, activated monocytes, and lymphoblasts in childhood leukemia. Endoglin is a component of the transforming growth factor-beta (TGFB) receptor complex and binds TGFB1 with high affinity.1 CD105 gene is mapped to 9q34.1. The coding region of the gene contains 14 exons.2 The protein consists of a homodimer of 180 kDA with disulfide links. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. Furthermore, it also has a role in the balance of ALK1 and ALK5 signaling to regulate endothelial cell proliferation.3 Moreover, the elevated expression of endoglin in the surgically excised CNVMs suggested a persisting postmitotic activation in an advanced stage of neovascular tissue.4 The standard product used in this kit is extracellular part of recombinant human CD105, from E26 to G586. As a result of glycosylation, the molecular mass is 75-85KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 312 285 280 306 295 13.53 4.5% Sample 2 1384 1301 1412 1238 1333 68.71 5.1% Sample 3 4821 5271 4779 5474 5086 295.48 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 312 285 280 306 295 13.53 4.5% Sample 1 312 285 280 306 295 13.53 4.5% Sample 2 1384 1301 1412 1238 1333 68.71 5.1% Sample 2 1384 1301 1412 1238 1333 68.71 5.1% Sample 3 4821 5271 4779 5474 5086 295.48 5.8% Sample 3 4821 5271 4779 5474 5086 295.48 5.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 312 1384 4821 316 1275 4955 Standard deviation 16.22 74.73 318.18 17.38 72.67 386.49 CV (%) 5.2% 5.4% 6.6% 5.5% 5.7% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 312 1384 4821 316 1275 4955 Standard deviation 16.22 74.73 318.18 17.38 72.67 386.49 CV (%) 5.2% 5.4% 6.6% 5.5% 5.7% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 312 1384 4821 316 1275 4955 Mean (pg/ml) 312 1384 4821 316 1275 4955 Standard deviation 16.22 74.73 318.18 17.38 72.67 386.49 Standard deviation 16.22 74.73 318.18 17.38 72.67 386.49 CV (%) 5.2% 5.4% 6.6% 5.5% 5.7% 7.8% CV (%) 5.2% 5.4% 6.6% 5.5% 5.7% 7.8%

Major glycoprotein of vascular endothelium. Involved in the regulation of angiogenesis. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Acts as TGF-beta coreceptor and is involved in the TGF- beta/BMP signaling cascade. Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGF-beta1 signaling through SMAD3. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.065 0.135 0.206 0.346 0.635 1.082 1.584 2.067 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.065 0.135 0.206 0.346 0.635 1.082 1.584 2.067 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.065 0.135 0.206 0.346 0.635 1.082 1.584 2.067 O.D. 0.065 0.135 0.206 0.346 0.635 1.082 1.584 2.067

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.