Human TSP2/THBS2/Thrombospondin-2 ELISA Kit PicoKine®

Human TSP2 ELISA Kit PicoKine® (96 Tests) . Quantitate Human THBS2 in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Thrombospondin-2 (TSP-2) is a protein that in humans is encoded by the THBS2 gene.And this gene is mapped to 6q27. The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration. TSP-2 has been shown to interact with MMP2.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 234 245 217 210 226 13.79 6.1% Sample 2 1702 1693 1719 1783 1724 35.18 2% Sample 3 6045 5875 6096 5562 5894 208.67 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 234 245 217 210 226 13.79 6.1% Sample 1 234 245 217 210 226 13.79 6.1% Sample 2 1702 1693 1719 1783 1724 35.18 2% Sample 2 1702 1693 1719 1783 1724 35.18 2% Sample 3 6045 5875 6096 5562 5894 208.67 3.5% Sample 3 6045 5875 6096 5562 5894 208.67 3.5%

Synonyms

Thrombospondin-2; THBS2; TSP2

Gene Name

THBS2

Gene ID

7058

UniProt

P35442

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: G19-I1172

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

High expression in invertebral disk tissue. .

Applications

ELISA

Standard

40 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.

Detection Range

625 pg/mL - 40,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 234 1702 6045 222 1773 6515 Standard deviation 17.55 789 411.06 17.76 117.54 501.65 CV (%) 7.5% 4.6% 6.8% 8% 6.5% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 234 1702 6045 222 1773 6515 Standard deviation 17.55 789 411.06 17.76 117.54 501.65 CV (%) 7.5% 4.6% 6.8% 8% 6.5% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 234 1702 6045 222 1773 6515 Mean (pg/ml) 234 1702 6045 222 1773 6515 Standard deviation 17.55 789 411.06 17.76 117.54 501.65 Standard deviation 17.55 789 411.06 17.76 117.54 501.65 CV (%) 7.5% 4.6% 6.8% 8% 6.5% 7.7% CV (%) 7.5% 4.6% 6.8% 8% 6.5% 7.7%

Sensitivity

<10 pg/mL

Function

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

129991 MW

Specificity

Natural and recombinant human TSP2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Thrombospondin-2

Sequence Similarities

Belongs to the thrombospondin family.

Protein Name

Thrombospondin-2

Sample Data

Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.004 0.025 0.054 0.116 0.254 0.6256 1.469 2.045 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.004 0.025 0.054 0.116 0.254 0.6256 1.469 2.045 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.004 0.025 0.054 0.116 0.254 0.6256 1.469 2.045 O.D. 0.004 0.025 0.054 0.116 0.254 0.6256 1.469 2.045

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.