Human Survivin ELISA Kit PicoKine®

Survivin (also termed API4) belongs to the family of genes known as inhibitors of apoptosis, and it has been implicated in both prevention of cell death and control of mitosis. Survivin is prominently expressed in transformed cell lines and in all the most common human cancers of lung, colon, pancreas, prostate and breast in vivo. It is also found in approximately 50% of high-grade non-Hodgkin's lymphomas. Survivin encodes a deduced 142-amino acid protein and localizes on chromosome 17q25. The overexpression of it in cancer may overcome an apoptotic checkpoint and favour aberrant progression of transformed cells through mitosis. The standard product used in this kit is recombinant human Survivin with the molecular mass of 34 KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 207 178 185 184 188 11.01 5.8% Sample 2 660 723 712 743 709 30.66 4.3% Sample 3 1259 1187 1079 1117 1160 68.8 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 207 178 185 184 188 11.01 5.8% Sample 1 207 178 185 184 188 11.01 5.8% Sample 2 660 723 712 743 709 30.66 4.3% Sample 2 660 723 712 743 709 30.66 4.3% Sample 3 1259 1187 1079 1117 1160 68.8 5.9% Sample 3 1259 1187 1079 1117 1160 68.8 5.9%

Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines. .

Epigenetics and Nuclear Signaling, Transcription, Apoptosis, Cancer, Cell Biology, Intracellular, Transcription Factors, Cell Cycle, Cell Death, Nuclear, Cell Division, Chromosome Structure, Invasion/Microenvironment

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 207 660 1259 192 690 1195 Standard deviation 15.52 48.18 51.61 14.13 57.27 66.92 CV (%) 7.5% 7.3% 4.1% 8.3% 8.3% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 207 660 1259 192 690 1195 Standard deviation 15.52 48.18 51.61 14.13 57.27 66.92 CV (%) 7.5% 7.3% 4.1% 8.3% 8.3% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 207 660 1259 192 690 1195 Mean (pg/ml) 207 660 1259 192 690 1195 Standard deviation 15.52 48.18 51.61 14.13 57.27 66.92 Standard deviation 15.52 48.18 51.61 14.13 57.27 66.92 CV (%) 7.5% 7.3% 4.1% 8.3% 8.3% 5.6% CV (%) 7.5% 7.3% 4.1% 8.3% 8.3% 5.6%

Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; s CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cytoplasm. Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Midbody. Localizes on chromosome arms and inner centromeres from prophase through metaphase. Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, localizes exclusively to the midbody. Colocalizes with AURKB at mitotic chromosomes. Acetylation at Lys-129 s its localization to the nucleus by enhancing homodimerization and thereby inhibiting XPO1/CRM1- mediated nuclear export.

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.039 0.142 0.239 0.372 0.617 1.014 1.566 2.098 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.039 0.142 0.239 0.372 0.617 1.014 1.566 2.098 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.039 0.142 0.239 0.372 0.617 1.014 1.566 2.098 O.D. 0.039 0.142 0.239 0.372 0.617 1.014 1.566 2.098

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.