Rat MMP-2 ELISA Kit PicoKine®

Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2 (MMP2) . It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown.1 Matrix metalloproteinases (MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide (cysteine switch) . The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability.2 The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization.3 The standard product used in this kit is recombinant rat MMP-2, consisting of 662 amino acids with the molecular mass of 72KDa. The detected MMP-2 includes zymogen and active enzyme.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1093 1090 975 1117 1068 55.12 5.1% Sample 2 4492 3849 4402 4495 4309 268.48 6.2% Sample 3 23073 23113 22919 26456 23890 1483.1 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1093 1090 975 1117 1068 55.12 5.1% Sample 1 1093 1090 975 1117 1068 55.12 5.1% Sample 2 4492 3849 4402 4495 4309 268.48 6.2% Sample 2 4492 3849 4402 4495 4309 268.48 6.2% Sample 3 23073 23113 22919 26456 23890 1483.1 6.2% Sample 3 23073 23113 22919 26456 23890 1483.1 6.2%

Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Cell Biology, Extracellular Matrix, Invasion/Microenvironment, Metabolism, Metabolism Processes, Metalloprotease, Pathways and Processes, Proteolysis/Ubiquitin, Proteolytic Enzymes, Response To Hypoxia, Tumor Biomarkers

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1093 4492 23073 1098 4440 21240 Standard deviation 68.85 296.47 1107.5 90.03 368.52 1296.64 CV (%) 8.3% 6.6% 4.8% 8.2% 8.3% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1093 4492 23073 1098 4440 21240 Standard deviation 68.85 296.47 1107.5 90.03 368.52 1296.64 CV (%) 8.3% 6.6% 4.8% 8.2% 8.3% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1093 4492 23073 1098 4440 21240 Mean (pg/ml) 1093 4492 23073 1098 4440 21240 Standard deviation 68.85 296.47 1107.5 90.03 368.52 1296.64 Standard deviation 68.85 296.47 1107.5 90.03 368.52 1296.64 CV (%) 8.3% 6.6% 4.8% 8.2% 8.3% 6.1% CV (%) 8.3% 6.6% 4.8% 8.2% 8.3% 6.1%

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Secreted, extracellular space, extracellular matrix . Membrane . Nucleus . Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes (By similarity) . .

Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.042 0.094 0.156 0.258 0.475 0.833 1.417 2.152 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.042 0.094 0.156 0.258 0.475 0.833 1.417 2.152 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.042 0.094 0.156 0.258 0.475 0.833 1.417 2.152 O.D. 0.042 0.094 0.156 0.258 0.475 0.833 1.417 2.152

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.