Human Prolactin ELISA Kit PicoKine®

Human Prolactin ELISA Kit PicoKine® (96 Tests) . Quantitate Human PRL in cell culture supernatants, serum and plasma (heparin, citrate) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Prolactin, also calle PRL, is a protein that in humans is encodes by the PRL gene. This gene is mapped to 6p22.3. This gene encodes the anterior pituitary hormone prolactin. It may play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor binding to cytokine like receptors it has also profound influence on hematopoiesis, angiogenesis and is involved in the regulation of blood clotting through several pathways.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 278 266 323 299 291 21.68 7.4% Sample 2 1989 1892 1944 1862 1921 48.66 2.5% Sample 3 4706 4695 5058 4926 4846 153.08 3.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 278 266 323 299 291 21.68 7.4% Sample 1 278 266 323 299 291 21.68 7.4% Sample 2 1989 1892 1944 1862 1921 48.66 2.5% Sample 2 1989 1892 1944 1862 1921 48.66 2.5% Sample 3 4706 4695 5058 4926 4846 153.08 3.1% Sample 3 4706 4695 5058 4926 4846 153.08 3.1%

Synonyms

Prolactin; PRL; PRL

Gene Name

PRL

Gene ID

5617

UniProt

P01236

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: L29-C227

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Growth Factors/Hormones, Lipid Metabolism, Metabolism, Protein Phosphorylation, Receptor Tyrosine Kinases, Signal Transduction, Tyrosine Kinases

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, citrate) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 278 1989 4706 274 1928 5020 Standard deviation 18.07 95.47 338.83 21.64 129.17 371.48 CV (%) 6.5% 4.8% 7.2% 7.9% 6.7% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 278 1989 4706 274 1928 5020 Standard deviation 18.07 95.47 338.83 21.64 129.17 371.48 CV (%) 6.5% 4.8% 7.2% 7.9% 6.7% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 278 1989 4706 274 1928 5020 Mean (pg/ml) 278 1989 4706 274 1928 5020 Standard deviation 18.07 95.47 338.83 21.64 129.17 371.48 Standard deviation 18.07 95.47 338.83 21.64 129.17 371.48 CV (%) 6.5% 4.8% 7.2% 7.9% 6.7% 7.4% CV (%) 6.5% 4.8% 7.2% 7.9% 6.7% 7.4%

Sensitivity

<10 pg/mL

Function

Prolactin acts primarily on the mammary gland by promoting lactation.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

25876 MW

Specificity

Natural and recombinant human Prolactin

Anticoagulant

Heparin or citrate

Gene Name Synonym

Prolactin

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the somatotropin/prolactin family.

Protein Name

Prolactin

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.057 0.119 0.152 0.252 0.461 0.792 1.326 2.153 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.057 0.119 0.152 0.252 0.461 0.792 1.326 2.153 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.057 0.119 0.152 0.252 0.461 0.792 1.326 2.153 O.D. 0.057 0.119 0.152 0.252 0.461 0.792 1.326 2.153

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.