Rat IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine®

Rat IL-18 ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Il18 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin (IL) -18

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 29 30 28 29 0.7 2.4% Sample 2 166 149 165 152 158 7.58 4.7% Sample 3 511 489 520 499 504 11.75 2.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 29 30 28 29 0.7 2.4% Sample 1 29 29 30 28 29 0.7 2.4% Sample 2 166 149 165 152 158 7.58 4.7% Sample 2 166 149 165 152 158 7.58 4.7% Sample 3 511 489 520 499 504 11.75 2.3% Sample 3 511 489 520 499 504 11.75 2.3%

Synonyms

Interleukin-18; IL-18; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma; Il18; Igif

Gene Name

IL18

Gene ID

29197

UniProt

P97636

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: H37-S194

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Angiogenesis, Cancer, Cancer Metabolism, Cytokines, Immunology, Innate Immunity, Interleukins, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways and Processes, Response To Hypoxia, Tumor Immunology

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 166 511 29 165 490 Standard deviation 1.97 2.2 22.99 2.2 9.07 29.4 CV (%) 6.8% 4.7% 4.5% 7.6% 5.5% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 166 511 29 165 490 Standard deviation 1.97 2.2 22.99 2.2 9.07 29.4 CV (%) 6.8% 4.7% 4.5% 7.6% 5.5% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 29 166 511 29 165 490 Mean (pg/ml) 29 166 511 29 165 490 Standard deviation 1.97 2.2 22.99 2.2 9.07 29.4 Standard deviation 1.97 2.2 22.99 2.2 9.07 29.4 CV (%) 6.8% 4.7% 4.5% 7.6% 5.5% 6% CV (%) 6.8% 4.7% 4.5% 7.6% 5.5% 6%

Sensitivity

<1 pg/mL

Function

Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

22303 MW

Specificity

Natural and recombinant rat IL-18

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin-18

Subcellular Location

Secreted.

Protein Name

Interleukin-18

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.007 0.064 0.128 0.224 0.456 0.842 1.354 1.973 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.007 0.064 0.128 0.224 0.456 0.842 1.354 1.973 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.007 0.064 0.128 0.224 0.456 0.842 1.354 1.973 O.D. 0.007 0.064 0.128 0.224 0.456 0.842 1.354 1.973

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.