Human TNF Beta/Lymphotoxin-alpha ELISA Kit PicoKine®

Tumor necrosis factor-beta (TNF-beta), previously called lymphotoxin-alpha (LTA), is a cytokine produced by lymphocytes. TNF-alpha and TNF-beta share 35% identity and 50% homology in the amino acid sequence. The substance LTA mediates a wide variety of inflammatory, immunostimulatory, and antiviral responses. LTA is associated with susceptibility to myocardial infarction, asthma and other diseases. The LTA gene is located on human chromosome 6. The standard product used in this kit is recombinant protein, consisting of 172 amino acids with the molecular mass of 18.8KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 165 162 154 142 155 8.89 5.7% Sample 2 487 454 458 413 453 26.37 5.8% Sample 3 1791 1778 1760 1730 1764 22.88 1.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 165 162 154 142 155 8.89 5.7% Sample 1 165 162 154 142 155 8.89 5.7% Sample 2 487 454 458 413 453 26.37 5.8% Sample 2 487 454 458 413 453 26.37 5.8% Sample 3 1791 1778 1760 1730 1764 22.88 1.2% Sample 3 1791 1778 1760 1730 1764 22.88 1.2%

Biochemicals, Chemical Type, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipid Signaling, Metabolic Signaling Pathways, Metabolism, Pathways and Processes

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 165 487 1791 179 521 1896 Standard deviation 11.71 32.14 127.16 13.06 38.55 157.36 CV (%) 7.1% 6.6% 7.1% 7.3% 7.4% 8.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 165 487 1791 179 521 1896 Standard deviation 11.71 32.14 127.16 13.06 38.55 157.36 CV (%) 7.1% 6.6% 7.1% 7.3% 7.4% 8.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 165 487 1791 179 521 1896 Mean (pg/ml) 165 487 1791 179 521 1896 Standard deviation 11.71 32.14 127.16 13.06 38.55 157.36 Standard deviation 11.71 32.14 127.16 13.06 38.55 157.36 CV (%) 7.1% 6.6% 7.1% 7.3% 7.4% 8.3% CV (%) 7.1% 6.6% 7.1% 7.3% 7.4% 8.3%

Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.053 0.103 0.142 0.237 0.404 0.813 1.47 2.373 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.053 0.103 0.142 0.237 0.404 0.813 1.47 2.373 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.053 0.103 0.142 0.237 0.404 0.813 1.47 2.373 O.D. 0.053 0.103 0.142 0.237 0.404 0.813 1.47 2.373

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.