Human Growth Hormone/GH1 ELISA Kit PicoKine®

Human Growth Hormone/GH1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human GH1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Growth hormone (GH) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Human growth hormone has a molecular mass of 22,005 and contains 191 amino acid residues with 2 disulfide bridges. The human genes for growth hormone (GH), has been located on chromosome 17 in humans. GH replacement of adults with acquired GH deficiency (GHD) results in body composition changes including increases in lean mass and bone mineral density. However, the effects of long-term GH therapy on cognitive function are largely unknown, and there are conflicting data regarding quality of life. The standard product used in this kit is recombinant human Growth Hormone, consisting of 192 amino acids with the molecular mass of 22KDa.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 29 31 29 29 0.86 2.9% Sample 2 192 199 200 197 197 3.08 1.5% Sample 3 394 427 443 440 426 19.42 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 29 31 29 29 0.86 2.9% Sample 1 29 29 31 29 29 0.86 2.9% Sample 2 192 199 200 197 197 3.08 1.5% Sample 2 192 199 200 197 197 3.08 1.5% Sample 3 394 427 443 440 426 19.42 4.5% Sample 3 394 427 443 440 426 19.42 4.5%

Synonyms

Growth hormone 1 isoform 1 ; HCG1749481, isoform CRA_s ; GH1 ; hCG_1749481

Gene Name

GH1

Gene ID

2688

UniProt

P01241

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: F27-F217

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Developmental Biology, Growth Factors/Hormones, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 192 394 28 188 423 Standard deviation 2.2 11.13 30.33 2.29 11.84 39.76 CV (%) 7.6% 5.8% 7.7% 8.2% 6.3% 9.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 192 394 28 188 423 Standard deviation 2.2 11.13 30.33 2.29 11.84 39.76 CV (%) 7.6% 5.8% 7.7% 8.2% 6.3% 9.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 29 192 394 28 188 423 Mean (pg/ml) 29 192 394 28 188 423 Standard deviation 2.2 11.13 30.33 2.29 11.84 39.76 Standard deviation 2.2 11.13 30.33 2.29 11.84 39.76 CV (%) 7.6% 5.8% 7.7% 8.2% 6.3% 9.4% CV (%) 7.6% 5.8% 7.7% 8.2% 6.3% 9.4%

Sensitivity

<2 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

24847 MW

Specificity

Natural and recombinant human GH1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Growth hormone 1

Subcellular Location

Secreted .

Protein Name

Somatotropin

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.033 0.111 0.179 0.301 0.574 0.89 1.5 2.085 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.033 0.111 0.179 0.301 0.574 0.89 1.5 2.085 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.033 0.111 0.179 0.301 0.574 0.89 1.5 2.085 O.D. 0.033 0.111 0.179 0.301 0.574 0.89 1.5 2.085

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.