Mouse CCL24/Eotaxin-2 ELISA Kit PicoKine®

Mouse CCL24/Eotaxin-2 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Ccl24 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-C motif) ligand 24 (CCL24) also known as myeloid progenitor inhibitory factor 2 (MPIF-2) or eosinophil chemotactic protein 2 (eotaxin-2) is a protein that in humans is encoded by the CCL24 gene.1 By use of PCR analysis of somatic cell hybrid DNAs, radiation hybrid mapping, and a chromosome 7-specific YAC library, mapped the SCYA24 gene to chromosome 7q11.23.2 CCL24 is a small cytokine belonging to the CC chemokine family. CCL24 interacts with chemokine receptor CCR3 to induce chemotaxis in eosinophils.3 This chemokine is also strongly chemotactic for resting T lymphocytes and slightly chemotactic for neutrophils.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 38 39 39 38 0.82 2.1% Sample 2 220 203 230 223 219 9.92 4.5% Sample 3 498 423 499 448 467 32.71 7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 38 39 39 38 0.82 2.1% Sample 1 37 38 39 39 38 0.82 2.1% Sample 2 220 203 230 223 219 9.92 4.5% Sample 2 220 203 230 223 219 9.92 4.5% Sample 3 498 423 499 448 467 32.71 7% Sample 3 498 423 499 448 467 32.71 7%

Synonyms

C-C motif chemokine 24; Eosinophil chemotactic protein 2; Eotaxin-2; Small-inducible cytokine A24; Ccl24; Scya24

Gene Name

CCL24

Gene ID

56221

UniProt

Q9JKC0

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V27-V119

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Highest expression in jejunum and spleen. Lower levels found in liver and lung. No expression detected in kidney, thymus, brain or testis. .

Applications

ELISA

Field of Research

Chemokines, Immunology, Innate Immunity

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 220 498 37 218 503 Standard deviation 2.55 10.12 35.85 2.55 12.42 38.73 CV (%) 6.9% 4.6% 7.2% 6.9% 5.7% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 220 498 37 218 503 Standard deviation 2.55 10.12 35.85 2.55 12.42 38.73 CV (%) 6.9% 4.6% 7.2% 6.9% 5.7% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 37 220 498 37 218 503 Mean (pg/ml) 37 220 498 37 218 503 Standard deviation 2.55 10.12 35.85 2.55 12.42 38.73 Standard deviation 2.55 10.12 35.85 2.55 12.42 38.73 CV (%) 6.9% 4.6% 7.2% 6.9% 5.7% 7.7% CV (%) 6.9% 4.6% 7.2% 6.9% 5.7% 7.7%

Sensitivity

<10 pg/mL

Function

Chemotactic for resting T-lymphocytes, and eosinophils. Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Binds to CCR3 (By similarity) . .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

12871 MW

Specificity

Natural and recombinant mouse CCL24

Anticoagulant

Heparin or EDTA

Gene Name Synonym

C-C motif chemokine 24

Subcellular Location

Secreted.

Protein Name

C-C motif chemokine 24

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.036 0.103 0.18 0.306 0.561 1.048 1.557 2.013 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.036 0.103 0.18 0.306 0.561 1.048 1.557 2.013 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.036 0.103 0.18 0.306 0.561 1.048 1.557 2.013 O.D. 0.036 0.103 0.18 0.306 0.561 1.048 1.557 2.013

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.