Human EG-VEGF/PK1/PROK1 ELISA Kit PicoKine®

EG-VEGF, Endocrine glandderived vascular endothelial growth factor, is a human protein encoded by by the PROKR1 gene. Its gene is mapped to chromosome 1. The 1.4-kb cDNA encodes a protein of 105 amino acids with a well defined signal sequence. The mature protein is predicted to have 86 amino acids. Northern blot analysis demonstrated expression in testis, ovary, adrenal gland, and placenta. EG-VEGF has played a vital role in inducing proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands. The standards of this kit are recombinant human EG-VEGF (A20-F105), with molecular weight of 10.5 KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 35 30 33 32 1.92 6% Sample 2 249 238 216 221 231 13.2 5.7% Sample 3 474 409 474 466 455 27.18 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 35 30 33 32 1.92 6% Sample 1 31 35 30 33 32 1.92 6% Sample 2 249 238 216 221 231 13.2 5.7% Sample 2 249 238 216 221 231 13.2 5.7% Sample 3 474 409 474 466 455 27.18 5.9% Sample 3 474 409 474 466 455 27.18 5.9%

Localizes to glandular epithelium, stroma and vascular epithelial cells of first trimester decidua (at protein level) . Up-regulated in first trimester decidua when compared with non-pregnant endometrium. Expressed in the steroidogenic glands, ovary, testis, adrenal and placenta. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 249 474 31 228 490 Standard deviation 1.45 18.17 30.81 1.61 18.92 39.2 CV (%) 4.7% 7.3% 6.5% 5.2% 8.3% 8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 249 474 31 228 490 Standard deviation 1.45 18.17 30.81 1.61 18.92 39.2 CV (%) 4.7% 7.3% 6.5% 5.2% 8.3% 8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 31 249 474 31 228 490 Mean (pg/ml) 31 249 474 31 228 490 Standard deviation 1.45 18.17 30.81 1.61 18.92 39.2 Standard deviation 1.45 18.17 30.81 1.61 18.92 39.2 CV (%) 4.7% 7.3% 6.5% 5.2% 8.3% 8% CV (%) 4.7% 7.3% 6.5% 5.2% 8.3% 8%

Potently contracts gastrointestinal (GI) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types. Induces proliferation and differentiation, but not migration, of enteric neural crest cells. ly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. Positively regulates PTGS2 expression and prostaglandin synthesis. May play a role in placentation. May play a role in normal and pathological testis angiogenesis. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.032 0.137 0.228 0.414 0.74 1.402 2.049 2.447 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.032 0.137 0.228 0.414 0.74 1.402 2.049 2.447 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.032 0.137 0.228 0.414 0.74 1.402 2.049 2.447 O.D. 0.032 0.137 0.228 0.414 0.74 1.402 2.049 2.447

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.