Human CD40 Ligand/TNFSF5/CD40LG ELISA Kit PicoKine®

CD40 ligand (CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb/IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells.CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 203 178 187 209 194 12.35 6.3% Sample 2 762 793 661 730 736 48.95 6.6% Sample 3 1128 1109 1044 1131 1103 35.09 3.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 203 178 187 209 194 12.35 6.3% Sample 1 203 178 187 209 194 12.35 6.3% Sample 2 762 793 661 730 736 48.95 6.6% Sample 2 762 793 661 730 736 48.95 6.6% Sample 3 1128 1109 1044 1131 1103 35.09 3.1% Sample 3 1128 1109 1044 1131 1103 35.09 3.1%

CD40 ligand; CD40-L; T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5; CD154; CD40 ligand, membrane form; CD40 ligand, soluble form; CD40LG; CD40L, TNFSF5, TRAP

Adaptive Immunity, Atherosclerosis, Cardiovascular, Cytokines, Immunoglobulins, Immunology, Innate Immunity, Platelets, T Cells, TNF Superfamily, Vascular Inflammation

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 203 762 1128 198 762 1161 Standard deviation 15.42 58.1%8 56.4 17.02 61.72 77.78 CV (%) 7.6% 7.4% 5% 8.6% 8.1% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 203 762 1128 198 762 1161 Standard deviation 15.42 58.1%8 56.4 17.02 61.72 77.78 CV (%) 7.6% 7.4% 5% 8.6% 8.1% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 203 762 1128 198 762 1161 Mean (pg/ml) 203 762 1128 198 762 1161 Standard deviation 15.42 58.1%8 56.4 17.02 61.72 77.78 Standard deviation 15.42 58.1%8 56.4 17.02 61.72 77.78 CV (%) 7.6% 7.4% 5% 8.6% 8.1% 6.7% CV (%) 7.6% 7.4% 5% 8.6% 8.1% 6.7%

Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.063 0.151 0.225 0.372 0.623 0.981 1.518 2.046 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.063 0.151 0.225 0.372 0.623 0.981 1.518 2.046 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.063 0.151 0.225 0.372 0.623 0.981 1.518 2.046 O.D. 0.063 0.151 0.225 0.372 0.623 0.981 1.518 2.046

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.