Mouse CD30L ELISA Kit PicoKine®

Mouse CD30L ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Tnfsf8 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 6pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

The human CD30L (CD153) gene is located at 9q33. It contains 4 exons and spans more than 17.1 kb. CD153 is expressed on the surface of B cells and this expression is upregulated upon CD154 (CD40LG), IL4, and B-cell receptor engagement. In these cells, engagement of CD153 by T cell CD30 inhibits immunoglobulin class switch recognition as well as IgG, IgA, and IgE production, suggesting that thisreverse signaling modulates the CD154-dependent switching of B cells into the pool producing IgG, IgA, and IgE. Additionally, recombinant human CD30L enhanced the proliferation of CD3 -activated T cells, but induced differential responses, including cell death, in several CD30-positive lymphoma-derived cell lines.The standard product used in this kit is recombinant mouse CD30L with the molecular mass of 30-45KDa.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 30 34 31 32 2.06 6.4% Sample 2 254 260 260 224 249 14.92 5.9% Sample 3 445 507 470 490 478 23.11 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 30 34 31 32 2.06 6.4% Sample 1 35 30 34 31 32 2.06 6.4% Sample 2 254 260 260 224 249 14.92 5.9% Sample 2 254 260 260 224 249 14.92 5.9% Sample 3 445 507 470 490 478 23.11 4.8% Sample 3 445 507 470 490 478 23.11 4.8%

Synonyms

Tumor necrosis factor ligand superfamily member 8; CD30 ligand; CD30-L; CD153; Tnfsf8; Cd30l, Cd30lg

Gene Name

TNFSF8

Gene ID

21949

UniProt

P32972

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q68-D239

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Adaptive Immunity, Immunology, T Cells

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 254 445 38 267 477 Standard deviation 2.24 16.51 34.26 2.58 21.09 43.4 CV (%) 6.4% 6.5% 7.7% 6.8% 7.9% 9.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 254 445 38 267 477 Standard deviation 2.24 16.51 34.26 2.58 21.09 43.4 CV (%) 6.4% 6.5% 7.7% 6.8% 7.9% 9.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 254 445 38 267 477 Mean (pg/ml) 35 254 445 38 267 477 Standard deviation 2.24 16.51 34.26 2.58 21.09 43.4 Standard deviation 2.24 16.51 34.26 2.58 21.09 43.4 CV (%) 6.4% 6.5% 7.7% 6.8% 7.9% 9.1% CV (%) 6.4% 6.5% 7.7% 6.8% 7.9% 9.1%

Sensitivity

<6 pg/mL

Function

Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

26519 MW

Specificity

Natural and recombinant mouse CD30L

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Tumor necrosis factor ligand superfamily member 8

Subcellular Location

Membrane; Single-pass type II membrane protein.

Protein Name

Tumor necrosis factor ligand superfamily member 8

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.054 0.101 0.146 0.221 0.382 0.804 1.401 2.073 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.054 0.101 0.146 0.221 0.382 0.804 1.401 2.073 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.054 0.101 0.146 0.221 0.382 0.804 1.401 2.073 O.D. 0.054 0.101 0.146 0.221 0.382 0.804 1.401 2.073

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.