Mouse CD30/TNFRSF8 ELISA Kit PicoKine®

Mouse CD30/TNFRSF8 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Tnfrsf8 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. This gene is mapped to 1p36.22. CD30 is expressed in embryonal carcinoma but not in seminoma and is thus a useful marker in distinguishing between these germ cell tumors. CD30 mast cell activation represents an IgE-independent activation pathway, which is important for understanding cutaneous inflammation associated with mast cells. In addition to those, CD30 is also associated with anaplastic large cell lymphoma.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 35 38 39 38 1.87 4.9% Sample 2 255 232 265 245 249 12.21 4.9% Sample 3 414 393 421 433 415 14.53 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 35 38 39 38 1.87 4.9% Sample 1 40 35 38 39 38 1.87 4.9% Sample 2 255 232 265 245 249 12.21 4.9% Sample 2 255 232 265 245 249 12.21 4.9% Sample 3 414 393 421 433 415 14.53 3.5% Sample 3 414 393 421 433 415 14.53 3.5%

Synonyms

Tumor necrosis factor receptor superfamily member 8; CD30L receptor; Lymphocyte activation antigen CD30; CD30; Tnfrsf8

Gene Name

TNFRSF8

Gene ID

21941

UniProt

Q60846

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: F19-T281

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Detected in thymus and in activated splenocytes.

Applications

ELISA

Field of Research

Cancer, Cell Type Markers, Cytokines, Immunology, Innate Immunity, Receptors, TNF Superfamily, Tumor Biomarkers, Tumor Immunology

Standard

10 ng/Tube

Sample Type

Cell culture supernatants and serum.

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 255 414 42 257 418 Standard deviation 2.6 16.83 13.19 3.19 20.56 25.49 CV (%) 6.5% 6.6% 4.3% 7.6% 8% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 255 414 42 257 418 Standard deviation 2.6 16.83 13.19 3.19 20.56 25.49 CV (%) 6.5% 6.6% 4.3% 7.6% 8% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 40 255 414 42 257 418 Mean (pg/ml) 40 255 414 42 257 418 Standard deviation 2.6 16.83 13.19 3.19 20.56 25.49 Standard deviation 2.6 16.83 13.19 3.19 20.56 25.49 CV (%) 6.5% 6.6% 4.3% 7.6% 8% 6.1% CV (%) 6.5% 6.6% 4.3% 7.6% 8% 6.1%

Sensitivity

<10 pg/mL

Function

Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF- kappa-B (By similarity) . .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

53216 MW

Specificity

Natural and recombinant mouse CD30

Gene Name Synonym

Tumor necrosis factor receptor superfamily member 8

Subcellular Location

Membrane ; Single-pass type I membrane protein .

Protein Name

Tumor necrosis factor receptor superfamily member 8

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.001 0.063 0.129 0.322 0.62 1.124 1.697 2.059 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.001 0.063 0.129 0.322 0.62 1.124 1.697 2.059 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.001 0.063 0.129 0.322 0.62 1.124 1.697 2.059 O.D. 0.001 0.063 0.129 0.322 0.62 1.124 1.697 2.059

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.