Mouse MCP-1 ELISA Kit PicoKine®

Monocyte chemotactic protein-1 (MCP-1) is a member of the small inducible gene (SIG) family. It has been shown to play a role in the recruitment of monocytes to sites of injury and infection. By analysis of a panel of somatic cell hybrids, we have localized the MCP-1 gene, designated SCYA2, to human chromosome 17. In situ hybridization confirmed this assignment and further localized the gene to 17q11.2-q21.1. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Monocyte chemoattractant protein-1 (MCP-1), regulated on activation, normal T cell expressed and secreted, and stimulation of monocytes from healthy carriers of the genotype GG with Mycobacterium tuberculosis antigens yielded higher MCP-1. The standard used in this kit is recombinant mouse MCP-1 (Q24-R96) with the molecular mass of 8.5Kda.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 39 36 34 34 35 2.04 5.8% Sample 2 224 228 228 219 224 3.69 1.6% Sample 3 394 375 431 394 398 20.3 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 39 36 34 34 35 2.04 5.8% Sample 1 39 36 34 34 35 2.04 5.8% Sample 2 224 228 228 219 224 3.69 1.6% Sample 2 224 228 228 219 224 3.69 1.6% Sample 3 394 375 431 394 398 20.3 5.1% Sample 3 394 375 431 394 398 20.3 5.1%

C-C motif chemokine 2; Monocyte chemoattractant protein 1; Monocyte chemotactic protein 1; MCP-1; Platelet-derived growth factor-inducible protein JE; Small-inducible cytokine A2; Ccl2; Je, Mcp1, Scya2

Angiogenesis, Atherosclerosis, Cancer, Cancer Metabolism, Cardiovascular, Chemokines, Heart Disease, Immunology, Innate Immunity, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways and Processes, Response To Hypoxia, Vascular Inflammation

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 39 224 394 40 231 398 Standard deviation 1.56 10.75 27.18 2 12.47 27.46 CV (%) 4% 4.8% 6.9% 5% 5.4% 6.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 39 224 394 40 231 398 Standard deviation 1.56 10.75 27.18 2 12.47 27.46 CV (%) 4% 4.8% 6.9% 5% 5.4% 6.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 39 224 394 40 231 398 Mean (pg/ml) 39 224 394 40 231 398 Standard deviation 1.56 10.75 27.18 2 12.47 27.46 Standard deviation 1.56 10.75 27.18 2 12.47 27.46 CV (%) 4% 4.8% 6.9% 5% 5.4% 6.9% CV (%) 4% 4.8% 6.9% 5% 5.4% 6.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.013 0.092 0.18 0.34 0.642 1.107 1.836 2.559 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.013 0.092 0.18 0.34 0.642 1.107 1.836 2.559 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.013 0.092 0.18 0.34 0.642 1.107 1.836 2.559 O.D. 0.013 0.092 0.18 0.34 0.642 1.107 1.836 2.559

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.