Human CCL1/I 309 ELISA Kit PicoKine®

Human CCL1/I 309 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CCL1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 4pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CCL1, Chemokine (C-C motif) ligand 1, is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine receptor CCR8.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 28 28 28 28 0.86 3% Sample 2 196 177 181 179 183 7.49 4% Sample 3 432 458 433 415 434 15.33 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 28 28 28 28 0.86 3% Sample 1 30 28 28 28 28 0.86 3% Sample 2 196 177 181 179 183 7.49 4% Sample 2 196 177 181 179 183 7.49 4% Sample 3 432 458 433 415 434 15.33 3.5% Sample 3 432 458 433 415 434 15.33 3.5%

Synonyms

C-C motif chemokine 1; Small-inducible cytokine A1; T lymphocyte-secreted protein I-309; CCL1; SCYA1

Gene Name

CCL1

Gene ID

6346

UniProt

P22362

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: K24-K96

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Chemokines, Immunology, Innate Immunity

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 196 432 31 192 406 Standard deviation 2.28 11.36 31.53 2.51 11.9 32.07 CV (%) 7.6% 5.8% 7.3% 8.1% 6.2% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 196 432 31 192 406 Standard deviation 2.28 11.36 31.53 2.51 11.9 32.07 CV (%) 7.6% 5.8% 7.3% 8.1% 6.2% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 30 196 432 31 192 406 Mean (pg/ml) 30 196 432 31 192 406 Standard deviation 2.28 11.36 31.53 2.51 11.9 32.07 Standard deviation 2.28 11.36 31.53 2.51 11.9 32.07 CV (%) 7.6% 5.8% 7.3% 8.1% 6.2% 7.9% CV (%) 7.6% 5.8% 7.3% 8.1% 6.2% 7.9%

Sensitivity

<4 pg/mL

Function

Cytokine that is chemotactic for monocytes but not for neutrophils. Binds to CCR8. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

10992 MW

Specificity

Natural and recombinant human CCL1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

C-C motif chemokine 1

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the intercrine beta (chemokine CC) family.

Protein Name

C-C motif chemokine 1

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.028 0.071 0.127 0.249 0.473 0.84 1.495 2.082 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.028 0.071 0.127 0.249 0.473 0.84 1.495 2.082 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.028 0.071 0.127 0.249 0.473 0.84 1.495 2.082 O.D. 0.028 0.071 0.127 0.249 0.473 0.84 1.495 2.082

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.