Human Cardiotrophin-1 / CTF1 ELISA Kit PicoKine®

Cardiotrophin-1 (CT-1) is a member of the family of cytokines that includes leukemia inhibitory factor (LIF), ciliary neurotrophic factor (CNTF), oncostatin M (OSM), interleukin-6 (IL6), and interleukin-11 (IL11) . And the CT-1 gene is mapped to 1p21-p13. The human CT-1 protein contains 201 amino acids and shares 80% amino acid identity with the 203-amino acid mouse CT-1 sequence; however, unlike the mouse protein, human CT-1 has 2 rather than 1 cys and has no N-glycosylation site. Despite lacking a signal sequence, secreted CT-1 and mouse CT-1 induce cardiac myocyte hypertrophy in cell culture and bind to both mouse and human LIFR but not to OSMR. Furthermore, A 1.7-kb CT-1 transcript was detected at high levels in heart, skeletal muscle, prostate, and ovary. Low levels were detected in lung, kidney, pancreas, thymus, testis, and small intestine, with little or no expression detected in brain, placenta, spleen, colon, and peripheral blood leukocytes. And it was also observed strong expression in fetal lung and kidney.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 253 241 250 241 246 5.35 2.1% Sample 2 924 988 933 938 945 24.9 2.6% Sample 3 3655 4348 4273 3862 4034 286.76 7.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 253 241 250 241 246 5.35 2.1% Sample 1 253 241 250 241 246 5.35 2.1% Sample 2 924 988 933 938 945 24.9 2.6% Sample 2 924 988 933 938 945 24.9 2.6% Sample 3 3655 4348 4273 3862 4034 286.76 7.1% Sample 3 3655 4348 4273 3862 4034 286.76 7.1%

Highly expressed in heart, skeletal muscle, prostate and ovary. Lower levels in lung, kidney, pancreas, thymus, testis and small intestine. Little or no expression in brain, placenta, liver, spleen, colon or peripheral blood leukocytes.

Atherosclerosis, Cardiovascular, Cytokines, Diabetes-associated, Energy Metabolism, Energy Transfer Pathways, Hypertrophy, Immunology, Innate Immunity, Lipid Metabolism, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 253 924 3655 274 930 3410 Standard deviation 12.65 69.3 288.74 16.16 70.68 300.08 CV (%) 5% 7.5% 7.9% 5.9% 7.6% 8.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 253 924 3655 274 930 3410 Standard deviation 12.65 69.3 288.74 16.16 70.68 300.08 CV (%) 5% 7.5% 7.9% 5.9% 7.6% 8.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 253 924 3655 274 930 3410 Mean (pg/ml) 253 924 3655 274 930 3410 Standard deviation 12.65 69.3 288.74 16.16 70.68 300.08 Standard deviation 12.65 69.3 288.74 16.16 70.68 300.08 CV (%) 5% 7.5% 7.9% 5.9% 7.6% 8.8% CV (%) 5% 7.5% 7.9% 5.9% 7.6% 8.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.045 0.111 0.167 0.296 0.562 0.989 1.719 2.679 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.045 0.111 0.167 0.296 0.562 0.989 1.719 2.679 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.045 0.111 0.167 0.296 0.562 0.989 1.719 2.679 O.D. 0.045 0.111 0.167 0.296 0.562 0.989 1.719 2.679

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.