Human CCL21 / 6Ckine / Exodus 2 ELISA Kit PicoKine®

Chemokine (C-C motif) ligand 21 (CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine (because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine (SLC) . By somatic cell hybrid and radiation hybrid analyses, mapped the SCYA21 gene to 9p13. Chemokines are a family of proteins that leukocyte migration and activation to inflammatory stimuli. CXC chemokine ligand 13 (CXCL13), CC chemokine ligand 21 (CCL21), and CCL19 are constitutively expressed in secondary lymphoid organs, where they control the placement of lymphocytes and dendritic cells. It was demonstrate that the local expression of homeostatic chemokines in nonlymphoid organs, such as the lung, plays an important role in protective immune responses.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 100 109 108 95 103 5.78 5.6% Sample 2 709 697 706 724 709 9.72 1.3% Sample 3 1352 1517 1447 1423 1434 58.95 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 100 109 108 95 103 5.78 5.6% Sample 1 100 109 108 95 103 5.78 5.6% Sample 2 709 697 706 724 709 9.72 1.3% Sample 2 709 697 706 724 709 9.72 1.3% Sample 3 1352 1517 1447 1423 1434 58.95 4.1% Sample 3 1352 1517 1447 1423 1434 58.95 4.1%

C-C motif chemokine 21;6Ckine; Beta-chemokine exodus-2; Secondary lymphoid-tissue chemokine; SLC; Small-inducible cytokine A21; CCL21; SCYA21; UNQ784/PRO1600

Highly expressed in high endothelial venules of lymph nodes, spleen and appendix. Intermediate levels found in small intestine, thyroid gland and trachea. Low level expression in thymus, bone marrow, liver, and pancreas. Also found in tonsil, fetal heart and fetal spleen.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 100 709 1352 103 706 1475 Standard deviation 4 28.36 82.47 6.9 31.77 92.92 CV (%) 4% 4% 6.1% 6.7% 4.5% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 100 709 1352 103 706 1475 Standard deviation 4 28.36 82.47 6.9 31.77 92.92 CV (%) 4% 4% 6.1% 6.7% 4.5% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 100 709 1352 103 706 1475 Mean (pg/ml) 100 709 1352 103 706 1475 Standard deviation 4 28.36 82.47 6.9 31.77 92.92 Standard deviation 4 28.36 82.47 6.9 31.77 92.92 CV (%) 4% 4% 6.1% 6.7% 4.5% 6.3% CV (%) 4% 4% 6.1% 6.7% 4.5% 6.3%

Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.065 0.108 0.154 0.266 0.428 0.768 1.298 2.038 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.065 0.108 0.154 0.266 0.428 0.768 1.298 2.038 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.065 0.108 0.154 0.266 0.428 0.768 1.298 2.038 O.D. 0.065 0.108 0.154 0.266 0.428 0.768 1.298 2.038

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.