Human VEGFR1/sFLT1 ELISA Kit PicoKine®

SVEGFR1, also known as sFMS-related tyrosine kinase 1 (sFLT1) . Oncogene sFLT belongs to the src gene family and is related to oncogene ROS . Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. sFLT is mapped to 13q12. sVEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice. The standard product used in this kit is recombinant human sVEGFR1, consisting of 905 amino acids with the molecular mass of 100KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 237 212 228 228 226 9.01 3.9% Sample 2 1369 1318 1326 1352 1341 20.36 1.5% Sample 3 4420 4343 4598 4779 4535 168.51 3.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 237 212 228 228 226 9.01 3.9% Sample 1 237 212 228 228 226 9.01 3.9% Sample 2 1369 1318 1326 1352 1341 20.36 1.5% Sample 2 1369 1318 1326 1352 1341 20.36 1.5% Sample 3 4420 4343 4598 4779 4535 168.51 3.7% Sample 3 4420 4343 4598 4779 4535 168.51 3.7%

Vascular endothelial growth factor receptor 1; VEGFR-1;2.7.10.1; Fms-like tyrosine kinase 1; FLT-1; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; FLT; Vascular permeability factor receptor; FLT1; FLT, FRT, VEGFR1

Detected in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform 2 is strongly expressed in placenta. Isoform 3 is expressed in corneal epithelial cells (at protein level) . Isoform 3 is expressed in vascular smooth muscle cells (VSMC) . .

Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Growth Factors, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways and Processes, Protein Phosphorylation, Response To Hypoxia, Signal Transduction, Tyrosine Kinases

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 237 1369 4420 240 1352 4316 Standard deviation 9.71 106.78 203.32 11.28 131.14 202.85 CV (%) 4.1% 7.8% 4.6% 4.7% 9.7% 4.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 237 1369 4420 240 1352 4316 Standard deviation 9.71 106.78 203.32 11.28 131.14 202.85 CV (%) 4.1% 7.8% 4.6% 4.7% 9.7% 4.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 237 1369 4420 240 1352 4316 Mean (pg/ml) 237 1369 4420 240 1352 4316 Standard deviation 9.71 106.78 203.32 11.28 131.14 202.85 Standard deviation 9.71 106.78 203.32 11.28 131.14 202.85 CV (%) 4.1% 7.8% 4.6% 4.7% 9.7% 4.7% CV (%) 4.1% 7.8% 4.6% 4.7% 9.7% 4.7%

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts (in vitro) . Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Likewise, isoforms lacking a transmembrane domain, such as isoform 2, isoform 3 and isoform 4, may function as decoy receptors for VEGFA. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC and YES1, and may also phosphorylate CBL. Isoform 1 phosphorylates PLCG. Promotes phosphorylation of AKT1 at 'Ser-473'. Promotes phosphorylation of PTK2/FAK1. Isoform 7 has a truncated kinase domain; it increases phosphorylation of SRC at 'Tyr-418' by unknown means and promotes tumor cell invasion. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.012 0.076 0.126 0.235 0.469 0.973 1.864 2.669 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.012 0.076 0.126 0.235 0.469 0.973 1.864 2.669 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.012 0.076 0.126 0.235 0.469 0.973 1.864 2.669 O.D. 0.012 0.076 0.126 0.235 0.469 0.973 1.864 2.669

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.