Rat VEGF ELISA Kit PicoKine®

Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.The standard product used in this kit is recombinant rat VEGF164, which is a 25KDa single chain as well as a 50KDa dimer.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 28 31 28 29 1.22 4.2% Sample 2 243 226 269 260 249 16.46 6.6% Sample 3 389 381 403 431 401 19.02 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 28 31 28 29 1.22 4.2% Sample 1 29 28 31 28 29 1.22 4.2% Sample 2 243 226 269 260 249 16.46 6.6% Sample 2 243 226 269 260 249 16.46 6.6% Sample 3 389 381 403 431 401 19.02 4.7% Sample 3 389 381 403 431 401 19.02 4.7%

Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Growth Factors, Growth Factors/Hormones, Metabolism, Metabolism Processes, Pathways and Processes, Response To Hypoxia, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 243 389 31 234 368 Standard deviation 1.91 18.95 16.72 2.07 16.75 17.66 CV (%) 6.6% 7.8% 4.3% 6.7% 7.8% 4.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 243 389 31 234 368 Standard deviation 1.91 18.95 16.72 2.07 16.75 17.66 CV (%) 6.6% 7.8% 4.3% 6.7% 7.8% 4.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 29 243 389 31 234 368 Mean (pg/ml) 29 243 389 31 234 368 Standard deviation 1.91 18.95 16.72 2.07 16.75 17.66 Standard deviation 1.91 18.95 16.72 2.07 16.75 17.66 CV (%) 6.6% 7.8% 4.3% 6.7% 7.8% 4.8% CV (%) 6.6% 7.8% 4.3% 6.7% 7.8% 4.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.052 0.129 0.202 0.319 0.568 0.98 1.661 2.361 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.052 0.129 0.202 0.319 0.568 0.98 1.661 2.361 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.052 0.129 0.202 0.319 0.568 0.98 1.661 2.361 O.D. 0.052 0.129 0.202 0.319 0.568 0.98 1.661 2.361

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.