Mouse VCAM-1 ELISA Kit PicoKine®

Vascular cell adhesion molecule 1 (VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis.Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention.Integrin alpha4beta1 (VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization.The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization.The standard product used in this kit is recombinant mouse VCAM-1, a dimeric protein linking with a disulfide bond. It consists of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 101.9KDa. As a result of glycosylation, the molecular mass of 120KDa is revealed by SDS-PAGE.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 371 349 375 317 353 23.02 6.5% Sample 2 1198 1269 1179 1267 1228 40.31 3.2% Sample 3 4387 4360 4345 3970 4265 171.26 4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 371 349 375 317 353 23.02 6.5% Sample 1 371 349 375 317 353 23.02 6.5% Sample 2 1198 1269 1179 1267 1228 40.31 3.2% Sample 2 1198 1269 1179 1267 1228 40.31 3.2% Sample 3 4387 4360 4345 3970 4265 171.26 4% Sample 3 4387 4360 4345 3970 4265 171.26 4%

Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue. Expressed in the bone marrow. .

Atherosclerosis, Cancer, Cardiovascular, Cell Adhesion, Cell Adhesion Molecules, Cytoskeleton/ECM, Invasion/Microenvironment, Mesenchymal Stem Cells, Neural Signal Transduction, Neurology Process, Neuroscience, Signal Transduction, Stem Cells, Surface Molecules, Vascular Inflammation

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 371 1198 4387 354 1298 4119 Standard deviation 26.8%2 86.25 298.31 29.02 112.92 317.16 CV (%) 6.8% 7.2% 6.8% 8.2% 8.7% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 371 1198 4387 354 1298 4119 Standard deviation 26.8%2 86.25 298.31 29.02 112.92 317.16 CV (%) 6.8% 7.2% 6.8% 8.2% 8.7% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 371 1198 4387 354 1298 4119 Mean (pg/ml) 371 1198 4387 354 1298 4119 Standard deviation 26.8%2 86.25 298.31 29.02 112.92 317.16 Standard deviation 26.8%2 86.25 298.31 29.02 112.92 317.16 CV (%) 6.8% 7.2% 6.8% 8.2% 8.7% 7.7% CV (%) 6.8% 7.2% 6.8% 8.2% 8.7% 7.7%

Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.001 0.082 0.174 0.333 0.609 0.997 1.508 2.112 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.001 0.082 0.174 0.333 0.609 0.997 1.508 2.112 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.001 0.082 0.174 0.333 0.609 0.997 1.508 2.112 O.D. 0.001 0.082 0.174 0.333 0.609 0.997 1.508 2.112

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.