Human VCAM-1 ELISA Kit PicoKine®

Human VCAM-1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human VCAM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Vascular cell adhesion molecule 1 (VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis. Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention. Integrin alpha4beta1 (VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization. The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization. The standard product used in this kit is recombinant human VCAM-1, consisting of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 74KDa. As a result of glycosylation, the molecular mass of 90-95KDa is revealed by SDS-PAGE.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 310 273 296 273 288 15.79 5.4% Sample 2 1065 1111 1014 939 1032 63.84 6.1% Sample 3 5287 5539 5884 6073 5695 303.89 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 310 273 296 273 288 15.79 5.4% Sample 1 310 273 296 273 288 15.79 5.4% Sample 2 1065 1111 1014 939 1032 63.84 6.1% Sample 2 1065 1111 1014 939 1032 63.84 6.1% Sample 3 5287 5539 5884 6073 5695 303.89 5.3% Sample 3 5287 5539 5884 6073 5695 303.89 5.3%

Synonyms

Vascular cell adhesion protein 1; V-CAM 1; VCAM-1; INCAM-100; CD106; VCAM1; L1CAM

Gene Name

VCAM1

Gene ID

7412

UniProt

P19320

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: F25-E698

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.

Applications

ELISA

Field of Research

Atherosclerosis, Cancer, Cardiovascular, Cell Adhesion, Cell Adhesion Molecules, Cytoskeleton/ECM, Invasion/Microenvironment, Mesenchymal Stem Cells, Neural Signal Transduction, Neurology Process, Neuroscience, Signal Transduction, Stem Cells, Surface Molecules, Vascular Inflammation

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 310 1065 5287 304 1029 5603 Standard deviation 17.67 51.12 387.35 19.15 65.85 481.85 CV (%) 5.7% 4.8% 7.3% 6.4% 6.4% 8.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 310 1065 5287 304 1029 5603 Standard deviation 17.67 51.12 387.35 19.15 65.85 481.85 CV (%) 5.7% 4.8% 7.3% 6.4% 6.4% 8.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 310 1065 5287 304 1029 5603 Mean (pg/ml) 310 1065 5287 304 1029 5603 Standard deviation 17.67 51.12 387.35 19.15 65.85 481.85 Standard deviation 17.67 51.12 387.35 19.15 65.85 481.85 CV (%) 5.7% 4.8% 7.3% 6.4% 6.4% 8.6% CV (%) 5.7% 4.8% 7.3% 6.4% 6.4% 8.6%

Sensitivity

<10 pg/mL

Function

Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

81276 MW

Specificity

Natural and recombinant human VCAM-1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Vascular cell adhesion protein 1

Subcellular Location

Membrane; Single-pass type I membrane protein.

Sequence Similarities

Contains 7 Ig-like C2-type (immunoglobulin-like) domains.

Protein Name

Vascular cell adhesion protein 1

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.068 0.118 0.236 0.463 0.797 1.32 2.023 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.068 0.118 0.236 0.463 0.797 1.32 2.023 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.068 0.118 0.236 0.463 0.797 1.32 2.023 O.D. 0.008 0.068 0.118 0.236 0.463 0.797 1.32 2.023

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.