Human uPAR / URKR / uPA Receptor ELISA Kit PicoKine®

The urokinase-type plasminogen activator receptor (uPAR) is a key molecule in the regulation of cell-surface plasminogen activation and, as such, plays an important role in many normal as well as pathological processes. The cDNA for Mo3, an activation antigen expressed by human monocytes and myelomonocytic cell lines after stimulation by a variety of agents. Mo3 expression in vivo is associated predominantly with macrophages in inflammatory sites. It is a highly glycosylated protein of about 50 kD in monocytes where it is anchored to the plasma membrane by glycosyl-phosphatidylinositol linkage. The complete coding sequence of the cDNA has been found to encode 335 amino acids including a predicted signal peptide of 22 residues and a hydrophobic C-terminal portion. Mo3 is identical to the human receptor for the urokinase plasminogen activator. UPAR is a useful prognostic marker for biologically aggressive forms of endometrial cancer. PLAUR is located at chromosome 19q13.1-q13.2. The standard product used in this kit is recombinant human uPAR, consisting of 287 amino acids with the molecular mass of 31KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 162 174 158 168 165 6.06 3.6% Sample 2 487 514 466 509 494 19.09 3.8% Sample 3 1379 1232 1375 1306 1323 60.02 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 162 174 158 168 165 6.06 3.6% Sample 1 162 174 158 168 165 6.06 3.6% Sample 2 487 514 466 509 494 19.09 3.8% Sample 2 487 514 466 509 494 19.09 3.8% Sample 3 1379 1232 1375 1306 1323 60.02 4.5% Sample 3 1379 1232 1375 1306 1323 60.02 4.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 162 487 1379 146 478 1361 Standard deviation 10.69 37.49 66.19 12.11 37.28 85.74 CV (%) 6.6% 7.7% 4.8% 8.3% 7.8% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 162 487 1379 146 478 1361 Standard deviation 10.69 37.49 66.19 12.11 37.28 85.74 CV (%) 6.6% 7.7% 4.8% 8.3% 7.8% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 162 487 1379 146 478 1361 Mean (pg/ml) 162 487 1379 146 478 1361 Standard deviation 10.69 37.49 66.19 12.11 37.28 85.74 Standard deviation 10.69 37.49 66.19 12.11 37.28 85.74 CV (%) 6.6% 7.7% 4.8% 8.3% 7.8% 6.3% CV (%) 6.6% 7.7% 4.8% 8.3% 7.8% 6.3%

Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cell membrane . Cell projection, invadopodium membrane . Colocalized with FAP (seprase) preferentially at the cell surface of invadopodia membrane in a cytoskeleton-, integrin- and vitronectin-dependent manner. .

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.016 0.113 0.201 0.364 0.673 1.137 1.688 2.042 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.016 0.113 0.201 0.364 0.673 1.137 1.688 2.042 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.016 0.113 0.201 0.364 0.673 1.137 1.688 2.042 O.D. 0.016 0.113 0.201 0.364 0.673 1.137 1.688 2.042

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.