Human uPA / PLAU / URK ELISA Kit PicoKine®

Plasminogen activator, urokinase (PLAU, uPA) converts plasminogen to plasmin. Plasmin is involved in processing of amyloid precursor protein and degrades secreted and aggregated amyloid-beta, a hallmark of Alzheimer disease (AD) . Urokinase has a molecular mass of about 54 kD and is composed of 2 disulfide-linked chains, A and B, of molecular masses 18 kD and 33 kD, respectively. It localized on 10q24. uPA facilitates cell migration by localizing proteolisys on the cell surface and by inducing intracellular signalling pathways. In human vascular smooth muscle cell (VSMC), uPA stimulates migration via the uPA receptor (uPAR) signalling complex containing TYK2 and phosphatidylinositol 3-kinase (PI3-K) .

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 195 183 178 191 186 6.64 3.5% Sample 2 583 571 542 578 568 15.88 2.7% Sample 3 1373 1415 1322 1294 1351 46.55 3.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 195 183 178 191 186 6.64 3.5% Sample 1 195 183 178 191 186 6.64 3.5% Sample 2 583 571 542 578 568 15.88 2.7% Sample 2 583 571 542 578 568 15.88 2.7% Sample 3 1373 1415 1322 1294 1351 46.55 3.4% Sample 3 1373 1415 1322 1294 1351 46.55 3.4%

Urokinase-type plasminogen activator; U-plasminogen activator; uPA;3.4.21.73; Urokinase-type plasminogen activator long chain A; Urokinase-type plasminogen activator short chain A; Urokinase-type plasminogen activator chain B; PLAU

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1211 583 1373 211 536 1493 Standard deviation 8.77 39.06 74.14 12.87 44.48 103.01 CV (%) 4.5% 6.7% 5.4% 6.1% 8.3% 6.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1211 583 1373 211 536 1493 Standard deviation 8.77 39.06 74.14 12.87 44.48 103.01 CV (%) 4.5% 6.7% 5.4% 6.1% 8.3% 6.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1211 583 1373 211 536 1493 Mean (pg/ml) 1211 583 1373 211 536 1493 Standard deviation 8.77 39.06 74.14 12.87 44.48 103.01 Standard deviation 8.77 39.06 74.14 12.87 44.48 103.01 CV (%) 4.5% 6.7% 5.4% 6.1% 8.3% 6.9% CV (%) 4.5% 6.7% 5.4% 6.1% 8.3% 6.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.004 0.069 0.135 0.26 0.495 0.905 1.421 2.05 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.004 0.069 0.135 0.26 0.495 0.905 1.421 2.05 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.004 0.069 0.135 0.26 0.495 0.905 1.421 2.05 O.D. 0.004 0.069 0.135 0.26 0.495 0.905 1.421 2.05

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.