Human TRAIL / CD253 ELISA Kit PicoKine®

Human TRAIL / CD253 ELISA Kit PicoKine® (96 Tests) . Quantitate Human TNFSF10 in cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

In the field of cell biology, TNF-related apoptosis-inducing ligand (TRAIL), is a protein which functions as a ligand which induces the process of cell death called apoptosis. TRAIL has also been designated CD253 (cluster of differentiation 253) .Tumor necrosis factor (TNF) family cytokines function as prominent mediators of immune regulation and the inflammatory response. Most TNF family cytokines are expressed as type II transmembrane proteins, with homology confined to approximately 150 C-terminal residues. The TNF ligands interact with a parallel family of receptors. TRAIL binds to the death receptors DR4 (TRAIL-RI) and DR5 (TRAIL-RII) . The process of apoptosis is caspase-8-dependent. Caspase-8 activates downstream effector caspases including procaspase-3, -6, and -7, leading to activation of specific kinases. TRAIL also binds the receptors DcR1 and DcR2, which do not contain a cytoplasmic domain (DcR1) or contain a truncated death domain (DcR2) . The standard product used in this kit is recombinant human TRAIL with the molecular mass of 21KDa. It is expressed from the amino acids ectoderm T95--G28.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 38 35 35 42 37 2.87 7.7% Sample 2 184 187 185 189 186 1.92 1% Sample 3 416 469 398 390 418 30.77 7.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 38 35 35 42 37 2.87 7.7% Sample 1 38 35 35 42 37 2.87 7.7% Sample 2 184 187 185 189 186 1.92 1% Sample 2 184 187 185 189 186 1.92 1% Sample 3 416 469 398 390 418 30.77 7.3% Sample 3 416 469 398 390 418 30.77 7.3%

Synonyms

Tumor necrosis factor ligand superfamily member 10; Apo-2 ligand; Apo-2L; TNF-related apoptosis-inducing ligand; Protein TRAIL; CD253; TNFSF10; APO2L, TRAIL

Gene Name

TNFSF10

Gene ID

8743

UniProt

P50591

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: T95-G281

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Widespread; most predominant in spleen, lung and prostate.

Applications

ELISA

Field of Research

Apoptosis, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cell Death, Invasion/Microenvironment, Receptors

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and saliva.

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 38 184 416 34 175 377 Standard deviation 2.66 9.56 18.72 2.75 9.62 18.85 CV (%) 7% 5.2% 4.5% 8.1% 5.5% 5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 38 184 416 34 175 377 Standard deviation 2.66 9.56 18.72 2.75 9.62 18.85 CV (%) 7% 5.2% 4.5% 8.1% 5.5% 5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 38 184 416 34 175 377 Mean (pg/ml) 38 184 416 34 175 377 Standard deviation 2.66 9.56 18.72 2.75 9.62 18.85 Standard deviation 2.66 9.56 18.72 2.75 9.62 18.85 CV (%) 7% 5.2% 4.5% 8.1% 5.5% 5% CV (%) 7% 5.2% 4.5% 8.1% 5.5% 5%

Sensitivity

<1 pg/mL

Function

Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

32509 MW

Specificity

Natural and recombinant human TRAIL

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Tumor necrosis factor ligand superfamily member 10

Subcellular Location

Membrane ; Single-pass type II membrane protein .

Sequence Similarities

Belongs to the tumor necrosis factor family.

Protein Name

Tumor necrosis factor ligand superfamily member 10

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.042 0.13 0.205 0.343 0.598 1.024 1.671 2.326 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.042 0.13 0.205 0.343 0.598 1.024 1.671 2.326 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.042 0.13 0.205 0.343 0.598 1.024 1.671 2.326 O.D. 0.042 0.13 0.205 0.343 0.598 1.024 1.671 2.326

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.