Mouse sTNFsR II ELISA Kit PicoKine®

Tumor necrosis factor receptor 2 (TNFR2) is one of receptors of TNF. TNF has proinflammatory and immunosuppressive properties that may segregate at the level of the 2 TNF receptors (TNFRs), TNFR1 and TNFR2. The genes for TNFR1, a 55-kDa protein, and TNFR2, a 70-kDa protein, have been mapped to human chromosomes 12 (12pter-cen) and 1 (1pter-p32), respectively. TNFR2 was induced on glomerular endothelial cells of nephritic kidneys, and TNFR2 expression on intrinsic cells, but not leukocytes, was essential for glomerulonephritis and glomerular complement deposition. TNFR1 promotes systemic immune responses and renal T cell death, while intrinsic cell TNFR2 plays a critical role in complement-dependent tissue injury. Therefore, therapeutic blockade specifically of TNFR2 may be a promising strategy in the treatment of immune-mediated glomerulonephritis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 36 36 36 35 1.29 3.6% Sample 2 269 252 258 227 251 15.4 6.1% Sample 3 494 524 520 463 500 24.39 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 36 36 36 35 1.29 3.6% Sample 1 33 36 36 36 35 1.29 3.6% Sample 2 269 252 258 227 251 15.4 6.1% Sample 2 269 252 258 227 251 15.4 6.1% Sample 3 494 524 520 463 500 24.39 4.8% Sample 3 494 524 520 463 500 24.39 4.8%

Tumor necrosis factor receptor superfamily member 1B; Tumor necrosis factor receptor 2; TNF-R2; Tumor necrosis factor receptor type II; TNF-RII; TNFR-II; p75; p80 TNF-alpha receptor; CD120b; Tnfrsf1b; Tnfr-2, Tnfr2

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 269 494 34 273 477 Standard deviation 2.37 17.21 34.58 2.55 21.56 37.2 CV (%) 7.2% 6.4% 7% 7.5% 7.9% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 269 494 34 273 477 Standard deviation 2.37 17.21 34.58 2.55 21.56 37.2 CV (%) 7.2% 6.4% 7% 7.5% 7.9% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 33 269 494 34 273 477 Mean (pg/ml) 33 269 494 34 273 477 Standard deviation 2.37 17.21 34.58 2.55 21.56 37.2 Standard deviation 2.37 17.21 34.58 2.55 21.56 37.2 CV (%) 7.2% 6.4% 7% 7.5% 7.9% 7.8% CV (%) 7.2% 6.4% 7% 7.5% 7.9% 7.8%

Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2 (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.036 0.1 0.183 0.317 0.648 1.024 1.592 1.861 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.036 0.1 0.183 0.317 0.648 1.024 1.592 1.861 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.036 0.1 0.183 0.317 0.648 1.024 1.592 1.861 O.D. 0.036 0.1 0.183 0.317 0.648 1.024 1.592 1.861

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.