Human sTNFsR II ELISA Kit PicoKine®

Human sTNFsR II ELISA Kit PicoKine® (96 Tests) . Quantitate Human TNFRSF1B in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Tumor necrosis factor receptor p75 (TNF-R p75, or TNF-R2) is a 75-kDa type I transmembrane protein expressed predominantly on cells of hematopoietic lineage. TNF-R p75 belongs to the TNF receptor superfamily characterized by cysteine-rich extracellular regions composed of three to six disulfide-linked domains. The tumor necrosis factor receptor II (TNFRII) gene localizes to 1p36. 2, a genomic region characteristically deleted in neuroblastomas and other malignancies. In addition, TNFRII is the principal mediator of the effects of TNF on cellular immunity, and it may cooperate with TNFRI in the killing of nonlymphoid cells. The standard product used in this kit is recombinant human sTNFRII, consisting of 24-206 amino acid sequence with the molecular mass of 20KDa.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 16 16 17 16 16 0.43 2.6% Sample 2 58 61 58 56 58 1.78 3% Sample 3 235 274 227 275 252 21.93 8.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 16 16 17 16 16 0.43 2.6% Sample 1 16 16 17 16 16 0.43 2.6% Sample 2 58 61 58 56 58 1.78 3% Sample 2 58 61 58 56 58 1.78 3% Sample 3 235 274 227 275 252 21.93 8.7% Sample 3 235 274 227 275 252 21.93 8.7%

Synonyms

Tumor necrosis factor receptor superfamily member 1B; Tumor necrosis factor receptor 2; TNF-R2; Tumor necrosis factor receptor type II; TNF-RII; TNFR-II; p75; p80 TNF-alpha receptor; CD120b; Etanercept; Tumor necrosis factor receptor superfamily member 1b, membrane form; Tumor necrosis factor-binding protein 2; TBP-2; TBPII; TNFRSF1B; TNFBR, TNFR2

Gene Name

TNFRSF1B

Gene ID

7133

UniProt

P20333

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L23-D257

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Apoptosis, Associated Proteins, Cancer, Cell Biology, Cell Death, Growth Factors/Hormones, Receptors, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Detection Range

7.8 pg/mL - 500 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 16 58 235 14 55 233 Standard deviation 1 2.43 15.04 0.89 3.19 15.37 CV (%) 5.8%% 4.2% 6.4% 6.4% 5.8% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 16 58 235 14 55 233 Standard deviation 1 2.43 15.04 0.89 3.19 15.37 CV (%) 5.8%% 4.2% 6.4% 6.4% 5.8% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 16 58 235 14 55 233 Mean (pg/ml) 16 58 235 14 55 233 Standard deviation 1 2.43 15.04 0.89 3.19 15.37 Standard deviation 1 2.43 15.04 0.89 3.19 15.37 CV (%) 5.8%% 4.2% 6.4% 6.4% 5.8% 6.6% CV (%) 5.8%% 4.2% 6.4% 6.4% 5.8% 6.6%

Sensitivity

<2 pg/mL

Function

Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

48291 MW

Specificity

Natural and recombinant human sTNFsRII

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Tumor necrosis factor receptor superfamily member 1B

Subcellular Location

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Sequence Similarities

Contains 4 TNFR-Cys repeats.

Protein Name

Tumor necrosis factor receptor superfamily member 1B

Sample Data

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.0 0.078 0.151 0.286 0.531 0.967 1.664 2.399 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.0 0.078 0.151 0.286 0.531 0.967 1.664 2.399 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.0 0.078 0.151 0.286 0.531 0.967 1.664 2.399 O.D. 0.0 0.078 0.151 0.286 0.531 0.967 1.664 2.399

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.