Human TNFsR I ELISA Kit PicoKine®

TNFsRI (also known as Solube tumor necrosis factor receptor 1) is a cytokine receptor that binds tumor necrosis factors (TNF) . In contrast, the p60 (TNFRSF1A) and p80 (TNFRSF1B) TNFA receptors self-assemble through a distinct functional domain in the TNFR extracellular domain, termed the pre-ligand assembly domain (PLAD), in the absence of ligand. Deletion of the PLAD results in monomeric presentation of p60 or p80. Flow cytometric analysis showed that efficient TNFA binding depends on receptor self-assembly. They also found that other members of the TNF receptor superfamily, including the extracellular domains of TRAIL (TNFRSF10A), CD40, and FAS (TNFRSF6), all self-associate but do not interact with heterologous receptors. By Southern blot analysis of human/Chinese hamster somatic cell hybrid DNA, the TNFR1 gene was mapped to 12pter-cen. And by nonradioactive in situ hybridization that the type 1 receptor (the p55 TNF receptor) is encoded by a gene located on chromosome 12p13.2.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 10 10 9 9 9 0.5 5.5% Sample 2 54 52 56 55 54 1.47 2.7% Sample 3 219 194 207 198 204 9.6 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 10 10 9 9 9 0.5 5.5% Sample 1 10 10 9 9 9 0.5 5.5% Sample 2 54 52 56 55 54 1.47 2.7% Sample 2 54 52 56 55 54 1.47 2.7% Sample 3 219 194 207 198 204 9.6 4.7% Sample 3 219 194 207 198 204 9.6 4.7%

Tumor necrosis factor receptor superfamily member 1A; Tumor necrosis factor receptor 1; TNF-R1; Tumor necrosis factor receptor type I; TNF-RI; TNFR-I; p55; p60; CD120a; Tumor necrosis factor receptor superfamily member 1A, membrane form; Tumor necrosis factor-binding protein 1; TBPI; TNFRSF1A; TNFAR, TNFR1

Antiviral Signaling, Apoptosis, Associated Proteins, Cancer, Cell Biology, Cell Death, Growth Factors/Hormones, Immune System Diseases, Immunology, Receptors, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 10 54 219 10 59 203 Standard deviation 0.55 3.99 13.14 0.58 4.48 13.39 CV (%) 5.5% 7.4% 6% 5.8% 7.6% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 10 54 219 10 59 203 Standard deviation 0.55 3.99 13.14 0.58 4.48 13.39 CV (%) 5.5% 7.4% 6% 5.8% 7.6% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 10 54 219 10 59 203 Mean (pg/ml) 10 54 219 10 59 203 Standard deviation 0.55 3.99 13.14 0.58 4.48 13.39 Standard deviation 0.55 3.99 13.14 0.58 4.48 13.39 CV (%) 5.5% 7.4% 6% 5.8% 7.6% 6.6% CV (%) 5.5% 7.4% 6% 5.8% 7.6% 6.6%

Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate- specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cell membrane ; Single-pass type I membrane protein . Golgi apparatus membrane ; Single- pass type I membrane protein . Secreted . A secreted form is produced through proteolytic processing.

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.025 0.071 0.114 0.204 0.334 0.647 1.25 1.898 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.025 0.071 0.114 0.204 0.334 0.647 1.25 1.898 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.025 0.071 0.114 0.204 0.334 0.647 1.25 1.898 O.D. 0.025 0.071 0.114 0.204 0.334 0.647 1.25 1.898

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.