Mouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine®

Mouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Tnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Human TNF

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 18 17 19 18 0.7 3.8% Sample 2 50 54 54 47 51 2.94 5.7% Sample 3 299 294 293 285 292 5.01 1.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 18 17 19 18 0.7 3.8% Sample 1 18 18 17 19 18 0.7 3.8% Sample 2 50 54 54 47 51 2.94 5.7% Sample 2 50 54 54 47 51 2.94 5.7% Sample 3 299 294 293 285 292 5.01 1.7% Sample 3 299 294 293 285 292 5.01 1.7%

Synonyms

Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Tumor necrosis factor, membrane form; N-terminal fragment; NTF; Intracellular domain 1; ICD1; Intracellular domain 2; ICD2; C-domain 1; C-domain 2; Tumor necrosis factor, soluble form; Tnf; Tnfa, Tnfsf2

Gene Name

TNF

Gene ID

21926

UniProt

P06804

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: L80-L235

Capture Antibody

Monoclonal antibody from hamster

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Metabolism, Signal Transduction, Immunology, Cancer, Atherosclerosis, Cardiovascular, Growth Factors/Hormones, Growth Factors, Innate Immunity, Vascular Inflammation, Cytokines, TNF Superfamily

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 50 299 16 53 305 Standard deviation 0.82 3.9 22.12 0.91 4.39 24.4 CV (%) 4.6% 7.8% 7.4% 5.7% 8.3% 8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 50 299 16 53 305 Standard deviation 0.82 3.9 22.12 0.91 4.39 24.4 CV (%) 4.6% 7.8% 7.4% 5.7% 8.3% 8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 18 50 299 16 53 305 Mean (pg/ml) 18 50 299 16 53 305 Standard deviation 0.82 3.9 22.12 0.91 4.39 24.4 Standard deviation 0.82 3.9 22.12 0.91 4.39 24.4 CV (%) 4.6% 7.8% 7.4% 5.7% 8.3% 8% CV (%) 4.6% 7.8% 7.4% 5.7% 8.3% 8%

Sensitivity

<1 pg/mL

Function

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

25896 MW

Specificity

Natural and recombinant Mouse TNF alpha

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Tumor necrosis factor

Subcellular Location

Cell membrane; Single-pass type II membrane protein.

Protein Name

Tumor necrosis factor

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.072 0.124 0.217 0.377 0.685 1.164 1.893 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.072 0.124 0.217 0.377 0.685 1.164 1.893 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.072 0.124 0.217 0.377 0.685 1.164 1.893 O.D. 0.008 0.072 0.124 0.217 0.377 0.685 1.164 1.893

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.