Human TIMP-3 ELISA Kit PicoKine®

Human TIMP-3 ELISA Kit PicoKine® (96 Tests) . Quantitate Human TIMP3 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

The tissue inhibitors of metalloproteinases (TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12.1-q13.2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy (SFD) .

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 233 260 261 243 249 11.79 4.7% Sample 2 1357 1342 1373 1251 1330 47.33 3.5% Sample 3 4383 3655 4220 4320 4144 288.52 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 233 260 261 243 249 11.79 4.7% Sample 1 233 260 261 243 249 11.79 4.7% Sample 2 1357 1342 1373 1251 1330 47.33 3.5% Sample 2 1357 1342 1373 1251 1330 47.33 3.5% Sample 3 4383 3655 4220 4320 4144 288.52 6.9% Sample 3 4383 3655 4220 4320 4144 288.52 6.9%

Synonyms

Metalloproteinase inhibitor 3; Protein MIG-5; Tissue inhibitor of metalloproteinases 3; TIMP-3; TIMP3

Gene Name

TIMP3

Gene ID

7078

UniProt

P35625

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: C24-P211

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Angiogenesis, Apoptosis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Neuroscience, Protease Inhibitors, Proteolysis/Ubiquitin, Sensory System, Signal Transduction, Visual System

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 233 1357 4383 248 1474 4049 Standard deviation 18.21 92.27 271.74 21.08 120.86 327.96 CV (%) 7% 6.8% 6.2% 8.5% 8.2% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 233 1357 4383 248 1474 4049 Standard deviation 18.21 92.27 271.74 21.08 120.86 327.96 CV (%) 7% 6.8% 6.2% 8.5% 8.2% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 233 1357 4383 248 1474 4049 Mean (pg/ml) 233 1357 4383 248 1474 4049 Standard deviation 18.21 92.27 271.74 21.08 120.86 327.96 Standard deviation 18.21 92.27 271.74 21.08 120.86 327.96 CV (%) 7% 6.8% 6.2% 8.5% 8.2% 8.1% CV (%) 7% 6.8% 6.2% 8.5% 8.2% 8.1%

Sensitivity

<2 pg/mL

Function

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

24145 MW

Specificity

Natural and recombinant human TIMP-3

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Metalloproteinase inhibitor 3

Subcellular Location

Secreted, extracellular space, extracellular matrix.

Sequence Similarities

Belongs to the protease inhibitor I35 (TIMP) family.

Protein Name

Metalloproteinase inhibitor 3

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.029 0.144 0.276 0.518 0.876 1.409 1.924 2.059 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.029 0.144 0.276 0.518 0.876 1.409 1.924 2.059 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.029 0.144 0.276 0.518 0.876 1.409 1.924 2.059 O.D. 0.029 0.144 0.276 0.518 0.876 1.409 1.924 2.059

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.