Human TIMP-2 ELISA Kit PicoKine®

TIMP2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 249 258 275 291 268 16.11 6% Sample 2 1786 1798 1796 2014 1848 95.65 5.1% Sample 3 4399 5066 4506 5016 4746 297.19 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 249 258 275 291 268 16.11 6% Sample 1 249 258 275 291 268 16.11 6% Sample 2 1786 1798 1796 2014 1848 95.65 5.1% Sample 2 1786 1798 1796 2014 1848 95.65 5.1% Sample 3 4399 5066 4506 5016 4746 297.19 6.2% Sample 3 4399 5066 4506 5016 4746 297.19 6.2%

Angiogenesis, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Protease Inhibitors, Proteolysis/Ubiquitin, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 249 1786 4399 239 1654 4356 Standard deviation 17.43 94.65 325.52 16.73 112.47 361.54 CV (%) 6.4% 5.3% 7.4% 7% 6.8% 8.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 249 1786 4399 239 1654 4356 Standard deviation 17.43 94.65 325.52 16.73 112.47 361.54 CV (%) 6.4% 5.3% 7.4% 7% 6.8% 8.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 249 1786 4399 239 1654 4356 Mean (pg/ml) 249 1786 4399 239 1654 4356 Standard deviation 17.43 94.65 325.52 16.73 112.47 361.54 Standard deviation 17.43 94.65 325.52 16.73 112.47 361.54 CV (%) 6.4% 5.3% 7.4% 7% 6.8% 8.3% CV (%) 6.4% 5.3% 7.4% 7% 6.8% 8.3%

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.03 0.08 0.128 0.231 0.405 0.77 1.336 2.165 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.03 0.08 0.128 0.231 0.405 0.77 1.336 2.165 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.03 0.08 0.128 0.231 0.405 0.77 1.336 2.165 O.D. 0.03 0.08 0.128 0.231 0.405 0.77 1.336 2.165

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.