Mouse Thrombopoietin/TPO ELISA Kit PicoKine®

Thrombopoietin (TPO) is also called megakaryocyte growth and development factor (MGDF) . TPO, a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from committed progenitor cells, is believed to be the major physiological regulator of circulating platelet levels. Human, dog, and mouse cDNAs for MGDF are highly conserved and encode open reading frames for proteins of 353, 352, and 356 amino acids, respectively, including predicted signal peptides. MGDF is a novel cytokine that regulates megakaryocyte development and is a ligand for the MPL receptor. The human MGDF gene has been mapped to chromosome 3q26.3. The standard product used in this kit is gene recombinant mouse TPO, consisting of 335 amino acids with the molecular mass of 35KDa. As a result of glycosylation, the molecular mass is 75KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 92 87 100 93 93 4.63 4.9% Sample 2 307 267 309 301 296 17 5.7% Sample 3 851 915 849 807 855 38.58 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 92 87 100 93 93 4.63 4.9% Sample 1 92 87 100 93 93 4.63 4.9% Sample 2 307 267 309 301 296 17 5.7% Sample 2 307 267 309 301 296 17 5.7% Sample 3 851 915 849 807 855 38.58 4.5% Sample 3 851 915 849 807 855 38.58 4.5%

Thrombopoietin; C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand; Thpo

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 92 307 851 85 330 918 Standard deviation 6.53 20.87 47.65 6.8 27.39 55.08 CV (%) 7.1% 6.8% 5.6% 8% 8.3% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 92 307 851 85 330 918 Standard deviation 6.53 20.87 47.65 6.8 27.39 55.08 CV (%) 7.1% 6.8% 5.6% 8% 8.3% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 92 307 851 85 330 918 Mean (pg/ml) 92 307 851 85 330 918 Standard deviation 6.53 20.87 47.65 6.8 27.39 55.08 Standard deviation 6.53 20.87 47.65 6.8 27.39 55.08 CV (%) 7.1% 6.8% 5.6% 8% 8.3% 6% CV (%) 7.1% 6.8% 5.6% 8% 8.3% 6%

Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.004 0.064 0.118 0.227 0.409 0.761 1.343 2.061 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.004 0.064 0.118 0.227 0.409 0.761 1.343 2.061 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.004 0.064 0.118 0.227 0.409 0.761 1.343 2.061 O.D. 0.004 0.064 0.118 0.227 0.409 0.761 1.343 2.061

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.