Human TGF Alpha ELISA Kit PicoKine®

Human TGF Alpha ELISA Kit PicoKine® (96 Tests) . Quantitate Human TGFA in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Transforming growth factor alpha (TGF-alpha) is upregulated in some human cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects. TGFalpha stimulates neural cell proliferation in the adult injured brain. Transforming growth factor alpha gene (TGFA) maps to human chromosome 2 close to the breakpoint of the t (2;8) variant translocation in Burkitt lymphoma. Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells; the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic human TGF-alpha showed similar immunoreactivity when compared with rat TGF-alpha. Thus, the 50-amino acid TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 33 31 35 32 1.65 5.1% Sample 2 230 219 227 246 230 9.81 4.2% Sample 3 448 498 470 454 467 19.35 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 33 31 35 32 1.65 5.1% Sample 1 31 33 31 35 32 1.65 5.1% Sample 2 230 219 227 246 230 9.81 4.2% Sample 2 230 219 227 246 230 9.81 4.2% Sample 3 448 498 470 454 467 19.35 4.1% Sample 3 448 498 470 454 467 19.35 4.1%

Synonyms

Protransforming growth factor alpha; Transforming growth factor alpha; TGF-alpha; EGF-like TGF; ETGF; TGF type 1; TGFA

Gene Name

TGFA

Gene ID

7039

UniProt

P01135

Reactivity

Human

Cross Reactivity

There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V40-A89

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. .

Applications

ELISA

Field of Research

Angiogenesis, Cancer, Cardiovascular, Growth Factors, Growth Factors/Hormones, Invasion/Microenvironment, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 230 448 29 237 431 Standard deviation 1.98 16.56 34.49 2.4 18.96 34.04 CV (%) 6.4% 7.2% 7.7% 8.3% 8% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 230 448 29 237 431 Standard deviation 1.98 16.56 34.49 2.4 18.96 34.04 CV (%) 6.4% 7.2% 7.7% 8.3% 8% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 31 230 448 29 237 431 Mean (pg/ml) 31 230 448 29 237 431 Standard deviation 1.98 16.56 34.49 2.4 18.96 34.04 Standard deviation 1.98 16.56 34.49 2.4 18.96 34.04 CV (%) 6.4% 7.2% 7.7% 8.3% 8% 7.9% CV (%) 6.4% 7.2% 7.7% 8.3% 8% 7.9%

Sensitivity

<1 pg/mL

Function

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

17006 MW

Specificity

Natural and recombinant human TGF alpha

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Protransforming growth factor alpha

Subcellular Location

Transforming growth factor alpha: Secreted, extracellular space.

Protein Name

Protransforming growth factor alpha

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.025 0.11 0.191 0.343 0.605 0.977 1.63 2.067 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.025 0.11 0.191 0.343 0.605 0.977 1.63 2.067 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.025 0.11 0.191 0.343 0.605 0.977 1.63 2.067 O.D. 0.025 0.11 0.191 0.343 0.605 0.977 1.63 2.067

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.