Mouse P-Selectin / CD62P ELISA Kit PicoKine®

P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is stored in secretory granules and expressed at the plasma membrane after cell activation. It is known to play an important role in atherosclerosis. The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1 (PSGL-1) . The standard product used in this kit is recombinant mouse P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 42-709 amino acids sequence with the molecular mass of 99.1KDa. As a result of glycosylation, the molecular mass of 190-200KDa is revealed by SDS-PAGE.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 596 594 610 551 587 22.09 3.7% Sample 2 2016 2063 1888 1979 1986 64.18 3.2% Sample 3 7521 7765 7893 8387 7891 315.75 4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 596 594 610 551 587 22.09 3.7% Sample 1 596 594 610 551 587 22.09 3.7% Sample 2 2016 2063 1888 1979 1986 64.18 3.2% Sample 2 2016 2063 1888 1979 1986 64.18 3.2% Sample 3 7521 7765 7893 8387 7891 315.75 4% Sample 3 7521 7765 7893 8387 7891 315.75 4%

P-selectin; CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; CD62P; Selp; Grmp

Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

Adaptive Immunity, Angiogenesis, Atherosclerosis, Cardiovascular, Cell Adhesion Molecules, Cell Type Markers, Hematopoietic Progenitors, Immunology, Myeloid, Platelets, Stem Cells, Vascular Inflammation

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 596 2016 7521 640 1834 7467 Standard deviation 28.6 106.84 443.73 32 105% 500.28 CV (%) 4.8% 5.3% 5.9% 5% 5.9% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 596 2016 7521 640 1834 7467 Standard deviation 28.6 106.84 443.73 32 105% 500.28 CV (%) 4.8% 5.3% 5.9% 5% 5.9% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 596 2016 7521 640 1834 7467 Mean (pg/ml) 596 2016 7521 640 1834 7467 Standard deviation 28.6 106.84 443.73 32 105% 500.28 Standard deviation 28.6 106.84 443.73 32 105% 500.28 CV (%) 4.8% 5.3% 5.9% 5% 5.9% 6.7% CV (%) 4.8% 5.3% 5.9% 5% 5.9% 6.7%

Ca (2+) -dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.025 0.195 0.311 0.514 0.801 1.312 1.763 2.029 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.025 0.195 0.311 0.514 0.801 1.312 1.763 2.029 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.025 0.195 0.311 0.514 0.801 1.312 1.763 2.029 O.D. 0.025 0.195 0.311 0.514 0.801 1.312 1.763 2.029

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.