Mouse sL-Selectin ELISA Kit PicoKine®

L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL (L-selectin) is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1L-selectin acts as a homing receptor for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 190 188 188 191 189 1.29 0.6% Sample 2 621 646 683 628 644 24.02 3.7% Sample 3 1771 1866 1906 1738 1820 68.25 3.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 190 188 188 191 189 1.29 0.6% Sample 1 190 188 188 191 189 1.29 0.6% Sample 2 621 646 683 628 644 24.02 3.7% Sample 2 621 646 683 628 644 24.02 3.7% Sample 3 1771 1866 1906 1738 1820 68.25 3.7% Sample 3 1771 1866 1906 1738 1820 68.25 3.7%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 190 621 1771 204 669 1831 Standard deviation 9.5 32.29 138.13 11.01 34.78 157.46 CV (%) 5% 5.2% 7.8% 5.4% 5.2% 8.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 190 621 1771 204 669 1831 Standard deviation 9.5 32.29 138.13 11.01 34.78 157.46 CV (%) 5% 5.2% 7.8% 5.4% 5.2% 8.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 190 621 1771 204 669 1831 Mean (pg/ml) 190 621 1771 204 669 1831 Standard deviation 9.5 32.29 138.13 11.01 34.78 157.46 Standard deviation 9.5 32.29 138.13 11.01 34.78 157.46 CV (%) 5% 5.2% 7.8% 5.4% 5.2% 8.6% CV (%) 5% 5.2% 7.8% 5.4% 5.2% 8.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.064 0.135 0.209 0.316 0.538 0.887 1.365 1.938 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.064 0.135 0.209 0.316 0.538 0.887 1.365 1.938 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.064 0.135 0.209 0.316 0.538 0.887 1.365 1.938 O.D. 0.064 0.135 0.209 0.316 0.538 0.887 1.365 1.938

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.