Human sL-Selectin ELISA Kit PicoKine®

L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL (L-selectin) is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1L-selectin acts as a homing receptor for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 195 209 190 190 196 7.77 3.9% Sample 2 543 572 564 574 563 12.27 2.1% Sample 3 1239 1413 1184 1268 1276 84.65 6.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 195 209 190 190 196 7.77 3.9% Sample 1 195 209 190 190 196 7.77 3.9% Sample 2 543 572 564 574 563 12.27 2.1% Sample 2 543 572 564 574 563 12.27 2.1% Sample 3 1239 1413 1184 1268 1276 84.65 6.6% Sample 3 1239 1413 1184 1268 1276 84.65 6.6%

L-selectin; CD62 antigen-like family member L; Leukocyte adhesion molecule 1; LAM-1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LECAM1; Lymph node homing receptor; TQ1; gp90-MEL; CD62L; SELL; LNHR, LYAM1

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1187 543 1239 187 595 1352 Standard deviation 17.8%1 33.66 60.71 16.64 37.48 67.6 CV (%) 7.8% 6.2% 4.9% 8.9% 6.3% 5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1187 543 1239 187 595 1352 Standard deviation 17.8%1 33.66 60.71 16.64 37.48 67.6 CV (%) 7.8% 6.2% 4.9% 8.9% 6.3% 5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1187 543 1239 187 595 1352 Mean (pg/ml) 1187 543 1239 187 595 1352 Standard deviation 17.8%1 33.66 60.71 16.64 37.48 67.6 Standard deviation 17.8%1 33.66 60.71 16.64 37.48 67.6 CV (%) 7.8% 6.2% 4.9% 8.9% 6.3% 5% CV (%) 7.8% 6.2% 4.9% 8.9% 6.3% 5%

Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.027 0.123 0.217 0.409 0.78 1.237 1.674 1.933 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.027 0.123 0.217 0.409 0.78 1.237 1.674 1.933 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.027 0.123 0.217 0.409 0.78 1.237 1.674 1.933 O.D. 0.027 0.123 0.217 0.409 0.78 1.237 1.674 1.933

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.